Heterologous Proteins Expressed in Pichia pastoris

Protein	Comments Mode, amount,  signal sequence	References and Links
Bacteria
Bacillus licheniformis alpha-amylase	S, 2.5 g/L, SUC2	[53, 54]
Bacillus stearothermophilus D-alanine carboxypeptidase	S, 100 mg/L, native	[55]
Bordetella pertussis Pertussis pertactin (P69)	I, 3 g/L	[56]
Metarhizium anisopliae chymotrypsin	S, α-MF, active	322
Clostridium botulinum neurotoxin (BoNT) serotype A and B	I, 78 mg/L	[57]
Clostridium botulinum neurotoxin, serotype A	I, 450 mg/kg cells	308
Clostridium botulinum neurotoxin heavy chain fragment, serotype B	I, 390 mg/g	[58]
Clostridium botulinum neurotoxin serotype F	I, 240 mg/kg cells	307
Clostridium botulinum neurotoxin serotype A binding domain	I, 2.4 mg total	[59]
Clostridium tetani Tetanus toxin fragment C	I, 12 g/L	[60]
Escherichia coli acid phosphatase/phytase (appA2)	S, 28.9 U/mg	[61]
Escherichia coli β-Galactosidase	I, 2.0 ´ 10^3 U/mg	[62]
Escherichia coli β-D-glucuronidase	I	309
Escherichia coli β-Lactamase	I	[63]
Leishmania major cathepsin B-like protease	S, α-MF	[64]
Staphylococcus aureus staphylokinase	S, 50 mg/L, α-MF	[65]
Streptococcus equisimili streptokinase	I, 77 mg/L, S, 3000 IU/ml, active	66, 319]
Streptomyces subtilisin inhibitor	S	[67]
Streptomyces viridosporus T7A endoglucanase	S, 2.47 g/L total protein, α-MF	[68]
Streptomyces viridosporus T7A peroxidase	S, 2.47 g/L total protein, α-MF	[68]
Toxoplasma gondii SAG1 antigen	S, 12 mg/L, α-MF	[69]
Vibrio cholerae accessory cholera enterotoxin (Ace)	S, 7 mg/L, α-MF	[70]
Fungi
Alternaria alternata Alt a 1 allergen	S, α-MF	[71]
Alternaria alternata rAlt a 2 allergen	S, 180mg/L, α-MF, functional	326
Aspergillus awamori glucoamylase	S, 400 mg/L, native	72
Aspergillus awamori glucoamylase catalytic domain	S, 400 mg/L, PHO1	[73]
Aspergillus fumigatus Asp f 2 allergen	I, weak reactivity	323
Aspergillus fumigatus catalase L	S, 2.3 g/L, PHO1	[74]
Aspergillus fumigatus dipeptidyl peptidase IV (DPP IV)	S, PHO1	[75]
Aspergillus fumigatus dipeptidyl peptidase V (DPP V)	S, 0.15 mg/L, PHO1	[76]
Aspergillus fumigatus phytase	S, 730 mg/L, α-MF, active	320
Aspergillus giganteus a-sarcin ribotoxin	S, 1 mg/L, synthetic native, PHO1	[77]
Aspergillus niger β-glucosidase	S, 0.5 g/L, PHO1	313
Aspergillus niger phytase (phyA)	S, 65 U/ml, α-MF	[78]
Aspergillus niger endo-β-1,4-xylanase	S, 60 mg/L, SUC2, native	311
Barley glucosidase		345
Candida albicans MNT1 mannosyltransferase	S, 250 mg/L, α-MF, active	324
Candida antarctica Lipase B	S, α-MF, 25 mg/L, active	321
Candida guilliermondii xylose reductase gene (xylI)	I, 0.65 U/mg; S, 0.18 U/mg, α-MF	[79]
Candida rugosa lipase 1 (CRL)	S, 150 U/ml, α-MF	[80]
Fusarium solani pectate lyase (pelC)	S, 1 mg/L, PHO1	[81]
Fusarium solani pectate lyase (pelD)	S, native	[82]
Geotrichum candidum lipase isoenzymes	S, 60 mg/L, α-MF	[83]
Metarhizium anisopliae chymotrypsin	S, α-MF, active	322
Mucor pusillus rennin	S, active	310
Neurospora crassa NOP-1	I, 2.2 mg/L, PHO1, active	312
Phanerochaete chrysosporium glyoxal oxidase	S, 2g/L, α-MF, active	325
Phaeseolus vulgaris aspartic protease	See ref 45 in331
Phytophthora cryptogea β-cyptogein	S, 45 mg/L, PHO1	[84]
Pycnoporus cinnabarinus I-937 laccase	S, 8 mg/L, α-MF and endogenous, active	318
Rhynchosporium secalis avirulence gene product NIP1	I, 16 mg/L, active	350
Rhizopus oryzae (also other yeast and bacteria)lipase	S, 60 mg/L, α-MF, active(200U/ml)	[85, 389
Saccharomyces cerevisiae CUP1 promoter and Pichia genes	Copper-inducible	316
Saccharomyces cerevisiae invertase	S, 2.5 g/L, native	[86]
Saccharomyces cerevisiae Ktr1p	S, 400 mg/L, PHO1	[87]
Saccharomyces cerevisiae (α-1,2-mannosyltransferase)	S, 40 mg/L, PHO1	[87]
Schizophyllum commune vitamin B2-aldehyde-forming enzyme	S, 120 mg/L, α-MF	[88]
Trametes versicolor (white rot fungus) laccase (lccI)	S, 1.6 g/L, native and α-MF	[89], 314
Trichoderma harzianum β-(1-6)-glucanase	S, 9.3 mg/L	[90]
Trichoderma reesei cellobiohydrolyase I	S, α-MF, low activity	315
Trichoderma reesei cellobiohydrolyase Cel7A	active	327
Trichoderma reesei 1,2-α-kD-mannosidase	S, 17.5 mg/L, α-MF, active	317
Protists
Actinia equina(sea anemone)equistatin	S, 25 mg/L, α-MF	424
Chondrus crispus red alga hexose oxidase	I	[91]
Gracilariopsis lemaneiformis red alga a-1,4-glucan lyase (GLq1)	I	[92]
Plasmodium falciparum merozoite surface protein 1 (MSP-1)	S, 24 mg/L, α-MF	[93]
Plasmodium falciparum antigen Pfs48/45	I (1% total protein) or S, α-MF, inactive	448
Plasmodium falciparum subtilisin-like protease pfsub1	I, 0.5g/L	449
Plasmodium vivax apical membrane antigen I (AMA-1)	S, 50 mg/L, PHO1	[94]
Ryschosporium secalis avirulence gene product NIP1	I, 16 mg/L, PHO1, active	334
Reticulomyxa filosa (giant freshwater amoeba) a2, b2 tubulin isoforms	I, 400 mg/g	[95]
Trypanosoma cruzi acid α-mannosidase	S, 11.5 mg/L, native	[96]
Plants
Allium sativum (garlic) alliin lyase	I, 2.167 U/g	[97]
Apple 1-aminocyclo-propane-1-carboxylate(ACC) synthase	I, 10 mg/L, active	333
Arabidopsis thaliana NADH:nitrate reductase	I, 18 mg/g	[98,99]
Arabidopsis thaliana aldehyde oxidase	I, active	337
Arabidopsis thaliana AtJ2 cochaperone protein	250 mg/L,	350
Barley (Hordeum vulgare) sucrose fructan 6-fructosyl transferase	S, α-MF	[100]
Barley a-amylase 1	S, 50 mg/L, native	[101]
Barley a-amylase 2	S, 1 mg/L, native	[101]
Barley aleurone tissue a-glucosidase	S, α-MF	[102]
Sugar beet defensin, AX2	S, active	336
Cassava(Manihot esculenta) P-450	I, 30 nmol/L, active	328
Cellulomas fimi family 2A carbohydrate-binding module	S, α-MF, active	330
Coffee bean a-galactosidase	S, 400 mg/L, α-MF, active	[103, 340, 341]
Corn(Zea maize) nitrate reductase	I, 42 mg/L, active	342
Corn(Zea maize) cytokinin oxidase	S, native	[111]
Cowpea (Vigna unguiculata) aspartic protease	See ref 45 in 331
Cynara cardunculus (cardoon) cyprosin	S, 1 mg/L, native	[104]
Cynodon dactylon (Bermuda grass) Cyn d 1	S, 1.5 g/L, PHO1	[105,106]
Galanthus nivalis agglutinin	S, PHA-E, α-MF, 2 mg/L	[107, 346]
Grass group 1 allergins(β-expansins)	S, active	335
Hevea brasiliensis hydroxynitrile lyase	I, 22 g/L	[108]
Hevea brasiliensis Hev b 7 patatin-like allergen	S, 10 mg/L, α-MF	[109,110]
Horseradish peroxidase	S, active	344
Mustard trypsin inhibitor 2	S, 160 mg/L, α-MF	347
Oat phytochrome A, phA	I, 30 mg/g	[112,113]
Oat phytochrome A, phyA65 apoprotein	I, 20 mg/g	[114]
Olea europaea (olive tree) aeroallergen Ole e 1	S, 60 mg/L, α-MF	[115, 334, 391]
Pepper endo-b-1,4-glucanase cCel1	S, α-MF	[116]
Pepper endo-b-1,4-glucanase cCel2	S, native	[116]
Persea americana (avocado) prs a 1 major allergen	S, 50 mg/L, α-MF	[117]
Phaseolus vulgaris agglutinin(PHA)	S, 1 mg/L, native	[346]
Pisum sativum copper amine oxidase	S, 1.4 mg/L, active	338
Pisum sativum mitochondrial pyruvate dehydrogenase	I, active	343
Pokeweed antiviral protein	S, 12 mg/L, α-MF, active	348
Potato phytochrome B	I, 25 mg/g	[118]
Potato proteinase inhibitor II	S, α-MF, active	329
Ragweed allergen Amb a 6	S, 1 mg/L, α-MF	[119]
Rice α-amylase	S, α-MF	339
Soybean root nodule acid phosphatase	S, 10 mg/L, α-MF	[120]
Spinach glycolate oxidase	I, 250 U/g	[121, 122]
Spinach nitrate reductase	I, 420 mg/L, active	342
Spinach phosphoribulokinase	I, 0.5 mg/g	[123]
Timothy grass group I allergen	S, α-MF	[124]
Tomato prosystemin	I, very low yield	332
Tomato Lycopesicon esculentum Mill. LeMir (L. esculentum miraculin)	S, PHO1	[125]
Wheat lipid transfer protein	S, 720 mg/L, PHO1	[126]
Invertebrates
Achacina fulica Ferussac (giant African snail) achacin	S, 0.2 mg/L, native	[127]
Acyclostoma caninum (hookworm)	S, active	388
Aplysia californica (marine mollusk) ADP ribosyl cyclase	S, 300 mg/L, α-MF	[128, 361
Aqueora victoria (jellyfish) green fluorescent protein	I, S, PHA-E	[129]
Boophilus microplus (cattle tick) Bm 85	I, 2 g/L, effective	353
Boophilus microplus (cattle tick) Bm86	I, S*, 1.5 g/L, SUC2	[131, 132, 133]
Cockroach allergen, Bla g 4	S, 50 mg/L	[134]
Diabrotica virgifera virgifera(corn rootworm) cysteine proteinase	S, α-MF, active	357
Drosophila melanogaster double-stranded specific adenosine deaminase	I, active	362
Drosophila melanogaster tRNA-specific adenosine deaminase	I, 133 ug/L	356
Drosophila melanogaster angiotensin I-converting enzyme	S, 160 mg/L, α-MF	[135]
Drosophila melanogaster malonyl-CoA-sensitive carnitine palmitoyltransferase	I, active	355
Firefly luciferase	I (peroxisome)	[136]
GAVACÔ vaccine against cattle tick	S, 2.0 g/L	[137]
Haementeria ghilanii (South American leech) ghilanten	S, 10 mg/L, α-MF	[138]
Hirudo medicinalis (leech) hirudin	S, 1.5 g/L, α-MF	[139]
Honeybee odorant-binding protein (ASP2)	S, 150 mg/L, native	[140]
Honeybee olfactory protein	S, 0.2g/L, native	[305]
Antarctic krill euphauserase	S, 500 μg/L, α-MF, active	358
Nematode anticoagulant protein c2	Active	367
Nematode elastase inhibitors	S, active	390
Nippostrongylus brasiliensis (parasitic nematode) non-neuronal secreted acetylcholinesterase	S, 50mg/L, α-MF, active	[141, 354]
Panulirus argus(spiny lobster) cytochrome P-450	I, 20 pmol/mg microsomal prot., active	351
Shistosoma mansoni asparaginyl endopeptidase	S, α-MF, active	352
Snake venom α-bungarotoxin	S, 2 mg/L, α-MF	359
Spider dragline silk protein	I, 663 mg/L	[142]
Tick anticoagulant peptide	S, 1.7 g/L, PHO secretion sig.,active	[143
Yellowjacket and paper wasp venom allergen A5	S, 100 mg/L, α-MF	360
Vertebrates (non-human)
Bovine β-casein	I, 1 g/L	[150]
Bovine enterokinase catalytic domain	S, 6.3 mg/L, α-MF	[144]
Bovine follicle stimulating hormone β-subunit	S, 4 mg/ml, α-MF	[145]
Bovine IFN-omega 1	S, 4 mg/L, SUC2	[146]
Bovine interferon-stimulated gene product 17	S, 6 mg/L, α-MF, active	380
Bovine β-lactoglobulin	S, >1 g/L, α-MF	[151,152,153, 387]
Bovine lysozyme c2	S, 550 mg/L, native	[147]
Bovine opsin	S*, 0.3 mg/L, PHO1	[148]
Bovine pancreatic trypsin inhibitor (aprotinin)	S, 930 mg/L, α-MF	[149]
Bovine tissue-type plasminogen activator (tPA)	S, 1.1 mg/L, α-MF	[154]
Bovine transcobalamin	S, α-MF	[306]
Brushtail possum TNFα	S, α-MF	[155]
Bungarus fasciatus (snake) venom gland acetylcholinesterase	S, 2 mg/L, native	[156]
Cat Fel d 1 and albumin	S, 3.3 mg/L, PHO1	393
Chicken liver a-N-acetylgalactosaminidase	S, 11.6 mg/L, α-MF, PHO1	[157]
Electrophorus electricus acetylcholinesterase AchE type T	S, native	[158]
Hen lysozyme	S, 20 mg/L, α-MF	[159]
Herring antifreeze protein	S, α-MF, active	377
Mammalian amyloid precursor fusion protein	S, α-MF, active	378
Mammalian lipocalin allergen Bos d2	S, mg amounts, native	[160]
Mammalian α2,6-sialyltransferase	I and S, active	401
Mouse activin type II receptor	S, 5 mg/L, active	395
Mouse single chain antibody Fv fragments (sFv)	S, 250 mg/L, α-MF, PHO1	[169, 365, 366, 369, 371, 372
Mouse CC Chemokine	S, α-MF, active	394
Murine endostatin	S, 200 mg/L, α-MF	170
Mouse epidermal growth factor	S, 450 mg/L, α-MF	[162]
Mouse gelatinase B	S, 10 mg/L, α-MF	[163]
Mouse P-glycoprotein	I, 700ug/100mg memb. protein, active	376
Mouse Mdr3 P-glycoprotein	I (membrane-bound), 6 mg/mg	[166,167,168, 383]
Murine macrophage inflammatory protein-2 (MIP-2)	S, 40 mg/L, α-MF	[172]
Mouse lysosomal acid a-mannosidase	S, native	[164]
Murine Golgi mannosidase IA	S, PHO1	[171]
Mouse Apha-methylacyl-CoA racemase	I, active	375
Mouse 5HT5A 5-tryptamine receptor	S*, 40 pmol/mg, α-MF	[161]
Mouse sonic hedgehog protein	S, α-MF, 500 mg/L, active	392
Mouse major urinary protein complex (MUP)	S, 270 mg/L, native	[165]
Recombinant non-hydroxlated gelatins(based on rat and mouse collagen)	S, 14.8 g/L, α-MF	385
Ovine follicle stimulating hormone (oFSH)	S, 22 mg/L, α-MF	[173]
Porcine carboxypeptidase B	S 250 mg/L, native, active	384
Porcine follicle-stimulating hormone	S, 10 mg/L, PHO1	[174]
Porcine inhibitor of carbonic anhydrase (transferrin family)	S, 5 mg/L, α-MF	[175]
Porcine single-chain interleukin-12	S, 4 mg/L,α-MF, active	370
Porcine leukocyte 12-lipoxygenase	I	[176]
Rabbit intestinal peptide transporter (PepT1)	I	[177]
Rabbit intestinal peptide transporter (PEPT2)	I	[178]
Rabbit antibody: monoclonal single-chain Fv specific for recombinant human leukemia inhibitory factor	S, 100 mg/L, α-MF	[179]
Rabbit plasma cholesteryl ester transfer protein	S, PHO1	[180]
Rabbit testicular angiotensin converting enzyme	S, PHO1, native	[181]
Rat acetycholinesterase	S, 1 mg/L, native	[156]
Rat brain acetylcholinesterase T subunit	S, 100 U/L α-MF	[182]
Rat calretinin gene fragments	S, 200 mg/L, α-MF, functional	379
Rat carnitine palmitoyltransferase I and II	I, active	373, 386
Rat CD59	S, 100 mg/L, α-MF	381
Rat complement regulator, crry	S, α-MF	[183 , 381]
Rat Golgi sialoglycoprotein MG160	S, 10 mg/L, α-MF	[184]
Rat high-mobility group 1 (HMG 1)	S, 50 mg/L, α-MF	[185]
Rat intrinsic factor	S, 40 mg/L, α-MF, functional	447
Rat liver mitochondrial carnitine palmitoyl transferases I and II (CPTI and II)	I (mitochondria)	, 187]
Rat NO synthase reductase domain	I, 25 mg/L	[188]
Rat odorant-binding protein	S , 80 mg/L, α-MF, same as wild type	364
Rat peroxisomal multifunctional enzyme (perMFE-II)	I	[189]
Rat procathepsin B	S, 100 mg/L, α-MF	[190, 191]
Rat tumor necrosis factor-α	I, 10 mg/L, active	382
Sea raven type II antifreeze protein (SRAFP)	S, 30 mg/L, α-MF	[192, 193]
Shark 17 a-hydroxylase/C17,20-lyase	I	[194]
Syrian golden hamster prion protein PrP^c	I, <0.1 mg/L	[195]
Humans
a(1,3/4) fucosyltransferase	S, 30 mg/L, α-MF	[130]
a-1,2-mannosidase 1B w/o TM domain	S, α-MF	[196]
a-N-Acetylgalactosaminidase (a-NAGAL)	S, 11.6 mg/L, α-MF	[197]
Antibody: scFv (against ovarian carcinoma)-biotin mimetic peptide	S	[263, 264]
Antibody: scFv (against squamous carcinoma)	S, 50 mg/L, α-MF	[245]
α1-antitrypsin (α1-AT)	S, inulinase signal sequence	[198]
β2-adrenergic receptor	S*, 25 nmol/g, α-MF	[161]
ADAR1, ADAR2,ds-RNA-specific adenosine deaminases	I, 1 mg/L	[200]
Alzheimer’s disease amyloid precursor protein α, β, and γ-secretase products	S, PHO1	[201]
Alzheimer’s disease amyloid precursor protein, 2 domains	S, 24 mg/L, 0.1 mg/L, α-MF	[202]
Amyloid precursor-like protein 2 (APLP2)	S, 40 mg/L, α-MF	[203]
Amyloid precursor protein (APP)	S, 24 mg/L, PHO1	[204, 205]
Amyloid precursor proteins, rAPP695, rAPP770	S, 4.5 + 1 mg/L, native	[206]
Bile salt stimulated lipase	S, 300 mg/L, native, INV	[207]
Bivalent diabody against carcinoembryonic antigen (CEA), T-cell coreceptor CD3-cell coreceptor CD2	S, 1 mg/L, α-MF	[208]
c-kit receptor kinase domain	I, 0.2 mg/L	[209, 210]
Carcinoembryonic antigen	S, 20 mg/L, α-MF	[211, 406
Heart muscle carnitine palmitoyltransferase I (M-CPTI) and II (CPTII)	I (mitochondria), active	232, 430, 386, 186
Caspase-3	I, 1 mg/g	[212]
Cathepsin K	S, 38 mg/L, α-MF	[213, 214]
Cathepsin L propeptide	S, 10 mg/L, α-MF	[215, 216]
Cathepsin V	S, α-MF	[217]
Cathepsin X	S, 5 mg/L, α-MF, activable	417
CD14	S, 20 mg/L,α-MF	414
CD38	S, 455 mg/L, α-MF	[218]
CD40 ligand soluble form	S, 255 mg/L	[219]
Chimeric B7-2 antibody fusion protein	S, 15 mg/L, α-MF	[220]
Chorionic gonadotropin α subunit, β subunit, and αβheterodimer	S, 24 mg/L (a), 3mg/L (b), 16 mg/L (ab), α-MF, active	[221, 428]
chymase	S, 200ug/L, SUC2, active	418
Fibroblast collagenase (proMMP-1)	S, 2.3 mg/L, α-MF	[229]
Type III collagen (with prolyl 4-hydroxylase)	I, 15 mg/L	[288]
C1r complement serine proteinase	S, 1.5 mg/L, ,α-MF, active	434
Complement receptor type 1	S, 50 mg/L, α-MF, binds C3b	407
Cromer blood group antigen decay-accelerating factor	S, α-MF	[222]
Cytomegalovirus ppUL44 antigen	I, 0.1 mg/ml	[223]
Decay accelerating factor-DAF (CD55)-Echovirus-7-receptor	S, 6 mg/L, α-MF	[224]
Double-stranded RNA-specific editase I (hREDI)	I, 1 mg/L	[225]
Elastase	S, α-MF, active	403
Endostatin	S, 20-200 mg/L, α-MF	[170, 226, 397
Erythropoietin receptor variant	S, 200 mg/L	439
Fas ligand	S, 100 mg/L, α-MF or PHO1, active	[227, 431]
Fibrinogen, 143-411, 143-427	S, 100 mg/L, 75 mg/L, α-MF	[228]
Fibrinogen-420 aEC domain	S, α-MF	[230]
Α-1,3-fucosyltransferase VI	S, α-MF, active(3U/L)	415
β-1,4-galactosyltransferase	S, α-MF, active(0.6U/L)	415
Galβ1,3-glucoronosyltransferase(GlcAT-1)	I or S, α-MF,active	421
Gastric cathepsin E	S, 0.6 mg/L, native	[231]
Gelatinase B	S, 50 mg/L, α-MF, active	425
Gp17/GCDFP-15	S, 3 mg/L, α-MF, active	399
Gp130	S, α-MF, active	416
Granzyme B	S, 1 mg/L, α-MF, active	432
11β-hydroxysteroid dehydrogenase 1/carbonyl reductase	I, active	396
Insulin	S, synthetic signal	[233]
Insulin-like growth factor-1 (IGF-1)	S, 600 mg/L, α-MF	[234
Interferon gamma receptor cytoplasmic domain	I	[235]
Interleukin-17 (hIL-17)	S, 0.35 mg/L, α-MF	[236]
Intracellular proteinase inhibitor (PI-6)	I, 50 mg/L	[237]
Intrinsic factor	S, 40 mg/L, α-MF, functional	447
Kunitz-type protease inhibitor domain of protease nexin-2/Amyloid b-protein precursor	S, 1.0 g/L, α-MF	[238]
Leukaemia inhibitory factor (LIF)	S, 17 mg/L, α-MF	[239]
Lewis fucosyl transferase( Fuc TIII)	Provide details,add link	130
Lymphocyte surface antigen CD38	S, 400 mg/L, PHO1	[240]
Lysosomal a-Mannosidase	S, 83 mg/L, native	[241]
Lysozyme	S, 300 mg/L, α-MF, active	405, 420
Mast cell tryptase	S, 6.5 mg/L, α-MF	[242, 243]
Membrane Type 1 Matrix Metalloproteinase	S, 25mg/L, α-MF, active	423
MHC class II heterodimers (soluble form/HLA-DR2)	S, 400 mg/L, α-MF	[244]
Monoclonal single chain Fv	S, 50 mg/L, α-MF	[245]
Monocyte chemoattractant protein-1 (MCP-1)	S, 100 mg/L, native or α-MF	[246]
Monocyte chemotactic protein 3 (hMCP-3)	S, 1 mg/L, PHO1	[247]
Neural cell adhesion molecule (NCAM)	S, 50 mg/L, PHO1	[248]
Neutral endopeptidase(neprilysin)	S, 10 mg/L, α-MF	402
NonO nucleic acid binding protein	I (endoplasmic reticulum)	[249]
Oncostatin M(OSM)	S, 50 mg/L, α-MF, active	416
mu-Opioid receptor	S*, α-MF	[199]
Pancreatic a-amylase	S, 20 mg/L, α-MF	[250]
Pancreatic triglyceride lipase	S, 75 ml/L, PHO1	[251]
Papain nitrile hydratase	S, 5 mg/L, α-MF	[252]
Group-V Phospholipase-A2	S, α-MF, active	450
Placental alkaline phosphatase (PLAP)	S, 2 mg/L, PHO1	[253]
Placental protein-14 (PP-14)	S, α-MF	[254]
Single-chain urokinase-type plasminogen activator	S, 5 mg/L, pre Mucor pusillus rennin (MPR) signal	[273]
Plasminogen kringle 1-4	S, 17 mg/L, PHO1	[255]
Plasminogen kringles 1-4, angiostatin protein	S, 10% total protein, PHO1	[256]
Plasminogen kringle 2	S, 100 mg/L, α-MF, functional	419
Presenilin I	I, α-MF, active	368
Procarboxypeptidase A2	S, 180 mg/L, α-MF	[257]
Procathepsin B	S, 20 mg/L, a-MF	[258]
Procolipase	S, 30 mg/L, native	[259]
Prolyl 4-hydroxylase	I, α-MF, active	437
Protein kinase C interacting protein 1 (PKCI-1)	I, 0.25 mg/L	[260]
Type 2A Protein Phosphatase, subunit PR65/A Subunit PP2Ac	I, 5 mg/l I, 100 ug/l(toxic?) Active complex	433
Proteinase 3, Wegener's antigen	S, 670 mg/L, α-MF	[261]
Proteinase inhibitor 8	I, 15% total protein	[262]
Serum albumin(wild type and disease variants)	S, 3 g/L, native, in Phase III trials	[265,266, 267, 268, 404, 408, 426
Serum transferrin N-Lobe	S, 240 mg/L, α-MF	[269,270,271]
Sex steroid-binding protein	S, 4 mg/L, α-MF	[272]
Galβ1,3GalNAc α2,3-sialyltransferase	S, PHO1, inactive	429
α-2,6-sialyltransferase	S, α-MF, active	415
Sonic hedgehog variants	S, α-MF, much is trauncated, inactive	438
Thrombomodulin	S	[274]
Tissue factor extracellular domain	S, 10 mg/L, PHO1	[275]
Tissue kallikrein	S, 30 mg/L, α-MF	[276, 277]
Tissue-type plasminogen activator kringle 2 domain	S, 170 mg/L, α-MF	[278,279,280, 281,282,283]
TRAIL tumor necrosis factor	S, 25 mg/L, α-MF, functional	436
Transforming growth factor β receptor extracellular domain	S, 10 mg/L, α-MF	[284]
Tumor necrosis factor αTNF	I, 10 g/L	[285, 286]
Type 1 plasminogen activator inhibitor (PAI-1)	S, 3 mg/L, α-MF	[287]
UDP-galactose-4-epimerase	I, 800 mg/L, α-MF, functional	435
UDP-glucoronosyltransferase (UDP-1A6)	I, active	422
Urokinase-type plasminogen activator-annexin V chimeras	S, 600 IU/ml, pre Mucor pussilus rennin (MPR) signal	[289]
Vascular endothelial growth factor (VEGF165)	S, 40 mg/L, PHO1	[290]
Viruses
A/VICTORIA/3/75 Influenza virus neuraminidase head domain	S, 3 mg/ml, α-MF	[291, 292]
Bovine Herpes virus-1 glycoprotein D	S, 20 mg/L, α-MF	[293, 294]
Dengue virus type 1 structural gene recombinant E protein	S, PHO1, prM virus signal sequence	[295]
Hepatitis B virus surface antigen	I, 400 mg/L	[296, 297]
Hepatitis B virus surface antigen-HIV gp41 epitope chimera	I	[298]
Hepatitis B X protein	I, RSV-CAT reporter	444
Hepatitis C E.1.339 polypeptide	I, functional	440
Hepatitis E virus ORF3	I	[299]
Human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein, gp120 (ENV)	S, 20 mg/L, α-MF	[300]
HIV-1 gp41 ectodomain	S, α-MF, active	446
Influenza virus RNA Polymerase	I, 2mg/L, α-MF, active	442
Newcastle disease virus head protein	S, α-MF	445
Polyomavirus Large T antigen	I, 0.5 mg/L	[301]
Reovirus Lambda 1 core protein	I, 0.8 mg/L	[302]
Reovirus sigma 1 protein	I	[303]
Vaccinia virus complement control protein	S, 3 mg/L, α-MF	[304, 443]

I = Intracellular (with subcellular location),
S = Secreted,
S* = Secreted to plasma membrane.
Amounts are highest reported for particular protein.
Signal sequences: a-MF (S. cerevisiae a-mating factor);
PHO1 (P. pastoris acid phosphatase);
SUC2 (S. cerevisiae invertase).

References

1. Romanos, M. A., Scorer, C. A., and Clare, J. J. (1992) Foreign gene expression in yeast: a review. Yeast 8, 423-488.
2. Cregg, J. M., Vedvick, T. S., and Raschke, W. C. (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Bio/Technology 11, 905-910.
3. Romanos, M. (1995) Advances in the use of Pichia pastoris pastoris for high-level expression. Curr. Opin. Biotechnol. 6, 527-533.
4. Cregg, J. M. (1999) Expression in the methylotrophic yeast Pichia pastoris. In Gene Expression Systems (Fernandez, J. and Hoeffler, J., ed.), Academic Press, San Diego, CA, pp. 157-191.
5. Cregg, J. M. and Higgins, D. R. (1995) Production of foreign proteins in the yeast Pichia pastoris. Can. J. Bot. 73(Suppl. 1), S981-S987.
6. Sreekrishna, K., Brankamp, R. G., Kropp, K. E., Blankenship, D. T., Tsay, J. T., Smith, P. L., Wierschke, J. D., Subramaniam, A., and Birkenberger, L. A. (1997) Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190, 55-62.
7. Gellissen, G., and Hollenberg, C. P. (1997) Application of yeasts in gene expression studies: a comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis÷a review. Gene 190, 87-97.
8. Higgins, D. R. (1995) Overview of protein expression in Pichia pastoris. In Current Protocols in Protein Science, Supplement 2 (Wingfield, P. T., ed.), John Wiley and Sons, New York, pp. 5.7.1-5.7.16.
9. Sreekrishna, K. (1993) Strategies for optimizing protein expression and secretion in the methylotrophic yeast Pichia pastoris. In Industrial Microorganisms: Basic and Applied Molecular Genetics (Baltz, R. H., Hegeman, G. D., and Skatrud, P. L., ed.), American Society for Microbiology, Washington, DC, Chap 16, pp. 119-126.
10. Ogata, K., Nishikawa, H., and Ohsugi, M. (1969) A yeast capable of utilizing methanol. Agric. Biol. Chem. 33, 1519ö1520.
11. Wegner, G. (1990) Emerging applications of methylotrophic yeasts. FEMS Microbiol. Rev. 87, 279-284.
12. Ellis, S. B., Brust, P. F., Koutz, P. J., Waters, A. F., Harpold, M. M., and Gingeras, T. R. (1985) Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol. Cell. Biol. 5, 1111-1121.
13. Cregg, J. M., Barringer, K. J., Hessler, A. Y., and Madden, K. R. (1985) Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5, 3376ö3385.
14. Tschopp, J. F., Brust, P. F., Cregg, J. M., Stillman, C. A., and Gingeras, T. R. (1987) Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 15, 3859-3876.
15. Cregg, J. M., and Madden, K. R. (1987) Development of yeast transformation systems and construction of methanol-utilization-defective mutants of Pichia pastoris gene disruption. In Biological Research on Yeasts (Stewart, G. G., Russell, I., Klein, R. D., and Hiebsch, R. R., ed.), CRC Press, Boca Raton, FL, Vol. II, pp. 1-18.
16. Cregg, J. M., Madden, K. R., Barringer, K. J., Thill, G. P., and Stillman, C. A. (1989) Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol. Cell. Biol. 9, 1316-1323.
17. Koutz, P. J., Davis, G. R., Stillman, C., Barringer, K., Cregg, J. M., and Thill, G. (1989) Structural comparison of the Pichia pastoris alcohol oxidase genes. Yeast 5, 167-177.
18. Lee, J.-D., and Komagata, K. (1980) Taxonomic study of methanol-assimilating yeasts. J. Gen. Appl. Microbiol. 26, 133-158.
19. Veenhuis, M., van Dijken, J. P., and Harder, W. (1983) The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts. Adv. Microb. Physiol. 24, 1-82.
20. Couderc, R., and Baratti, J. (1980) Oxidation of methanol by the yeast Pichia pastoris: purification and properties of alcohol oxidase. Agric. Biol. Chem. 44, 2279ö2289.
21. Tschopp, J. F., Sverlow, G., Kosson, R., Craig, W., and Grinna, L. (1987) High level secretion of glycosylated invertase in the methylotrophic yeast Pichia pastoris. Bio/Technology 5, 1305-1308.
22. Barr, K. A., Hopkins, S. A., and Sreekrishna, K. (1992) Protocol for efficient secretion of HSA developed from Pichia pastoris. Pharm. Eng. 12, 48-51.
23. Cregg, J. M., Tschopp, J. F., Stillman, C., Siegel, R., Akong, M., Craig, W. S., Buckholz, R. G., Madden, K. R., Kellaris, P. A., Davis, G. R., Smiley, B. L., Cruze, J., Torregrossa, R., Velicelebi, G., and Thill, G. P. (1987) High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Bio/Technology 5, 479-485.
24. Chirulova, V., Cregg, J. M., and Meagher, M. M. (1997) Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fed batch fermentations. Enzyme Microb. Technol. 21, 277-283.
25. Larouche, Y., Storme, V., De Muetter, J., Messens, J., and Lauwereys, M. (1994) High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast Pichia pastoris. Bio/Technology 12, 1119-1124.
26. Clare, J. J., Romanos, M. A., Rayment, F. B., Rowedder, J. E., Smith, M. A.. Payne, M. M., Sreekrishna, K., and Henwood, C. A. (1991) Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205-212.
27. Waterham, H. R., Digan, M. E., Koutz, P. J., Lair, S. L., and Cregg, J. M. (1997) Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene 186, 37-44.
28. Sreekrishna, K., Nelles, L., Potenz, R., Cruze, J., Mazzaferro, P., Fish, W., Fuke, M., Holden, K., Phelps, D., Wood, P., and Parker, K. (1989) High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry 28, 4117-4125.
29. Clare, J. J., Rayment, F. B., Ballantine, S. P., Sreekrishna, K., and Romanos, M. A. (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Bio/Technology 9, 455-ö460.
30. Romanos, M. A., Clare, J. J., Beesley, K. M., Rayment, F. B., Ballantine, S. P., Makoff, A. J., Dougan, G., Fairweather, N. F., and Charles, I. G. (1991) Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological properties. Vaccine 9, 901-906.
31. Wung, J. L., and Gascoigne, N. R. (1996) Antibody screening for secreted proteins expressed in Pichia pastoris. Bio/Techniques 21, 808, 810, 812.
32. Trimble, R. B., Atkinson, P. H., Tschopp, J. F., Townsend, R. R., and Maley, F. (1991) Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris. J. Biol. Chem. 266, 22807-22817.
33. Scorer, C. A., Clare, J. J., McCombie, W. R., Romanos, M. A., and Sreekrishna, K. (1994) Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Bio/Technology 12, 181-184. 34. Higgins, D.R. and Cregg, J.M. (1998) Pichia Protocols, Methods in Molecular Biology, vol 103, Human, Totowa, NJ.
35. Cereghino, J.L. and Cregg, J.M. (1999) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev., in press.
36. Shen, S., Sulter, G., Jeffries, T.W. and Cregg, J.M. (1998) A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris. Gene 216, 93-102.
37. Gronwald W; Loewen MC; Lix B; Daugulis AJ; Sonnichsen FD; Davies PL; Sykes BD. (1998) The solution structure of type II antifreeze protein reveals a new member of the lectin family. Biochemistry, 37(14):4712-21.
38. Kirkitadze MD; Krych M; Uhrin D; Dryden DT; Smith BO; Cooper A; Wang X; Hauhart R; Atkinson JP; Barlow PN. (1999) Independently melting modules and highly structured intermodular junctions within complement receptor type 1. Biochemistry, 38(22):7019-31.
39. McAlister MS; Brown MH; Willis AC; Rudd PM; Harvey DJ; Aplin R; Shotton DM; Dwek RA; Barclay AN; Driscoll PC. (1998) Structural analysis of the CD5 antigen--expression, disulphide bond analysis and physical characterisation of CD5 scavenger receptor superfamily domain 1. European Journal of Biochemistry, 257(1):131-41.
40. Morgan WD; Birdsall B; Frenkiel TA; Gradwell MG; Burghaus PA; Syed SE; Uthaipibull C; Holder AA; Feeney J. (1999) Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1. Journal of Molecular Biology, 289(1):113-22.
41. Wiles AP; Shaw G; Bright J; Perczel A; Campbell ID; Barlow PN. (1997) NMR studies of a viral protein that mimics the regulators of complement activation. Journal of Molecular Biology, 272(2):253-65.
42. Denton H; Smith M; Husi H; Uhrin D; Barlow PN; Batt CA; Sawyer L. (1998) Isotopically labeled bovine beta-lactoglobulin for NMR studies expressed in Pichia pastoris. Protein Expression and Purification, 14(1):97-103.
43. Uhrinova S; Uhrin D; Denton H; Smith M; Sawyer L; Barlow PN. Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: secondary structure and topology of the native state is retained in a partially unfolded form. Journal of Biomolecular Nmr, 1998 Jul, 12(1):89-107.
44. Kim TR; Goto Y; Hirota N; Kuwata K; Denton H; Wu SY; Sawyer L; Batt CA. High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical properties. Protein Engineering, 1997 Nov, 10(11):1339-45.
45. Mine S; Ueda T; Hashimoto Y; Tanaka Y; Imoto T.High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements. Febs Letters, 1999 Apr 1, 448(1):33-7.
46. Wood MJ; Komives EA. Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation. Journal of Biomolecular Nmr, 1999 Feb, 13(2):149-59.
47. McAlister MS; Davis B; Pfuhl M; Driscoll PC.NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein Engineering, 1998 Oct, 11(10):847-53.
48. Nilsen SL; DeFord ME; Prorok M; Chibber BA; Bretthauer RK; Castellino FJ. High-level secretion in Pichia pastoris and biochemical characterization of the recombinant kringle 2 domain of tissue-type plasminogen activator. Biotechnology and Applied Biochemistry, 1997 Feb, 25 ( Pt 1):63-74.
49. Greenwald J; Le V; Corrigan A; Fischer W; Komives E; Vale W; Choe S. Characterization of the extracellular ligand-binding domain of the type II activin receptor. Biochemistry, 1998 Nov 24, 37(47):16711-8.
50. Greenwald J; Fischer WH; Vale WW; Choe S. Three-finger toxin fold for the extracellular ligand-binding domain of the type II activin receptor serine kinase. Nature Structural Biology, 1999 Jan, 6(1):18-22.
51. Raymond, C.K., Bukowski, T., Holderman, S.D., Ching, A.F.T., Vanaja, E., and Stamm, M.R. (1997) Development of the methylotrophic yeast, Pichia methanolica, for the expression of the 65-kilodalton isoform of human glutamate decarboxylase. Yeast 14, 11-23.
52. Raymond, C.K. (1999) Recombinant protein expression in Pichia methanolica. In Gene Expression Systems (Fernandez, J. and Hoeffler, J., ed.), Academic Press, San Diego, CA, pp. 193-209.
53. Montesino, R., Garcia, R., Quintero, O. and Cremata, J.A. (1998) Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 14, 197-207.
54. Paifer, E., Margolles, E., Cremata, J., Montesino, R., Herrera, L. and Delgado, J.M. (1994) Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences. Yeast 10, 1415-1419.
55. Despreaux, C.W. and Manning, R.F. (1993) The dacA gene of Bacillus stearothemophilus coding for D-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris. Gene 131, 35-41.
56. Romanos, M.A., Clare, J.J., Beesley, K.M., Rayment, F.B., Ballantine, S.P., Makoff, A.J., Dougan, G., Fairweather, N.F. and Charles, I.G. (1991) Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high-level production and immunological properties. Vaccine 9, 901ö906.
57. Smith, L.A. (1998) Development of recombinant vaccines for botulinum neurotoxin. Toxicon 36, 1539-1548.
58. Potter, K.J., Bevins, M.A., Vassilieva, E.V., Chiruvolu, V.R., Smith, T., Smith, L.A. and Meagher, M.M. (1998) Production and purification of the heavy-chain fragment C of botulinum neurotoxin, serotype B, expressed in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 13, 357-365.
59. Byrne, M.P., Smith, T.J., Montgomery, V.A. and Smith, L.A. (1998) Purification, potency, and efficacy of the botulinum neurotoxin type A binding domain from Pichia pastoris as a recombinant vaccine candidate. Infect. Immun. 66, 4817-4822.
60. Clare, J.J., Rayment, F.B., Ballantine, S.P., Sreekrishna, K. and Romanos, M.A. (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Biotechnology (NY) 9, 455-460.
61. Rodriguez, E., Han, Y. and Lei, X.G. (1999) Cloning, sequencing, and expression of an Escherichia coli acid phosphatase/phytase gene (appA2) isolated from pig colon. Biochem. Biophys. Res. Commun. 257, 117-123.
62. Tschopp, J.F., Brust, P.F., Cregg, J.M., Stillman, C.A. and Gingeras, T.R. (1987) Expression of the LacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 15, 3859-3876.
63. Waterham, H.R., Digan, M.E., Koutz, P.J., Lair, S.V. and Cregg, J.M. (1997) Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene 186, 37-44.
64. Chan, V.J., Selzer, P.M., McKerrow, J.H. and Sakanari, J.A. (1999) Expression and alteration of the S2 subsite of the Leishmania major cathepsin B-like cysteine protease. Biochem. J. 340, 113-117.
65. Miele, R.G., Prorok, M., Costa, V.A. and Castellino, F.J. (1999) Glycosylation of asparagine-28 of recombinant staphylokinase with high-mannose-type oligosaccharides results in a protein with highly attenuated plasminogen activator activity. J. Biol. Chem. 274, 7769-7776.
66. Hagenson, M.J., Holden, K.A., Parker, K.A., Wood, P.J., Cruze, J.A., Fuke, M., Hopkins, T.R. and Stroman, D.W. (1989) Expression of streptokinase in Pichia pastoris yeast. Enzyme Microb. Technol. 11, 650-656.
67. Markaryan, A., Beall, C.J. and Kolattukudy, P.E. (1996) Inhibition of Aspergillus serine proteinase by Streptomyces subtilisin inhibitor and high-level expression of this inhibitor in Pichia pastoris. Biochem. Biophys. Res. Commun. 220, 372-376.
68. Thomas, L. and Crawford, D.L. (1998) Cloning of clustered Streptomyces viridosporus T7A lignocellulose catabolism genes encoding peroxidase and endoglucanase and their extracellular expression in Pichia pastoris. Can. J. Microbiol. 44, 364-372.
69. Biemans, R., Gregoire, D., Haumont, M., Bosseloir, A., Garcia, L., Jacquet, A., Dubeaux, C. and Bollen, A. (1998) The conformation of purified Toxoplasma gondii SAG1 antigen, secreted from engineered Pichia pastoris, is adequate for serorecognition and cell proliferation. J. Biotechnol. 66, 137-146.
70. Trucksis, M., Conn, T.L., Fasano, A. and Kaper, J.B. (1997) Production of Vibrio cholerae accessory cholera enterotoxin (Ace) in the yeast Pichia pastoris. Infect. Immun. 65, 4984-4988.
71. De Vouge, M.W., Thaker, A.J., Curran, I.H., Zhang, L., Muradia, G., Rode, H. and Vijay, H.M. (1996) Isolation and expression of a cDNA clone encoding an Alternaria alternata Alt a 1 subunit. Int. Arch. Allergy Immunol. 111, 385-395.
72. Fierobe, H.-P., Mirgorodskaya, E., Frandsen, T.P., Roepstorff, P. and Svensson, B. (1997) Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergillus niger as hosts. Protein Expr. Purif. 9, 159-170.
73. Heimo, H., Palmu, K. and Suominen, I. (1997) Expression in Pichia pastoris and purification of Aspergillus awamori glucoamylase catalytic domain. Protein Expr. Purif. 11, 304.
74. Calera, J.A., Paris, S., Monod, M., Hamilton, A.J., Debeaupuis, J.P., Diaquin, M., Lopez-Medrano, R., Leal, F. and Latge, J.P. (1997) Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus. Infect. Immun. 65, 4718-4724.
75. Beauvais, A., Monod, M., Wyniger, J., Debeaupuis, J.P., Grouzmann, E., Brakch, N., Svab, J., Hovanessian, A.G. and Latge, J.P. (1997) Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans. Infect. Immun. 65, 3042-3047.
76. Beauvais, A., Monod, M., Debeaupuis, J.P., Diaquin, M., Kobayashi, H. and Latge, J.P. (1997) Biochemical and antigenic characterization of a new dipeptidyl-peptidase isolated from Aspergillus fumigatus. J. Biol. Chem. 272, 6238-6244.
77. Martínez-Ruiz, A., Martínez del Pozo, A., Lacadena, J., Mancheño, J.M., Oñaderra, M., López-Otin, C. and Gavilanes, J.G. (1998) Secretion of recombinant pro- and mature fungal ?-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the LysöArg motif is required for maturation. Protein Expr. Purif. 12, 315-322.
78. Han, Y. and Lei, X.G. (1999) Role of glycosylation in the functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris. Arch. Biochem. Biophys. 364, 83-90.
79. Handumrongkul, C., Ma, D.P. and Silva, J.L. (1998) Cloning and expression of Candida guilliermondii xylose reductase gene (xyl1) in Pichia pastoris. Appl. Microbiol. Biotechnol. 49, 399-404
80. Brocca, S., Schmidt-Dannert, C., Lotti, M., Alberghina, L. and Schmid, R.D. (1998) Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase. Protein Sci. 7, 1415-1422.
81. Guo, W., Gonzalez-Candelas, L. and Kolattukudy, P.E. (1995) Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris. J. Bacteriol. 177, 7070-7077.
82. Guo, W., Gonzalez-Candelas, L. and Kolattukudy, P.E. (1996) Identification of a novel pelD gene expressed uniquely in planta by Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of its protein product as an endo-pectate lyase. Arch. Biochem. Biophys. 332, 305-312.
83. Holmquist, M., Tessier, D.C. and Cygler, M. (1997) High-level production of recombinant Geotrichum candidum lipases in yeast Pichia pastoris. Protein Expr. Purif. 11, 35-40.
84. O'Donohue, M.J., Boissy, G., Huet, J.C., Nespoulous, C., Brunie, S. and Pernollet, J.C. (1996) Overexpression in Pichia pastoris and crystallization of an elicitor protein secreted by the phytopathogenic fungus, Phytophthora cryptogea. Protein Expr. Purif. 8, 254-261.
85. Minning, S., Schmidt-Dannert, C. and Schmid, R.D. (1998) Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties. J. Biotechnol. 66, 147-156.
86. Tschopp, J.F., Sverlow, G., Kosson, R., Craig, W. and Grinna, L. (1987) High level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris. Bio/Technology 5, 1305-1308.
87. Romero, P.A., Lussier, M., Sdicu, A.M., Bussey, H. and Herscovics, A. (1997) Ktr1p is an alpha-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris. Biochem. J. 321, 289-295.
88. Chen, H. and McCormick, D.B. (1997) Riboflavin 5_-hydroxymethyl oxidation. Molecular cloning, expression, and glycoprotein nature of the 5_-aldehyde-forming enzyme from Schizophyllum commune. J. Biol. Chem. 272, 20077-20081.
89. Jonsson, L.J., Saloheimo, M. and Penttila, M. (1997) Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr. Genet. 32, 425-430.
90. Bom, I.J., Dielbandhoesing, S.K., Harvey, K.N., Oomes, S.J., Klis, F.M. and Brul, S. (1998) A new tool for studying the molecular architecture of the fungal cell wall: one-step purification of recombinant trichoderma beta-(1-6)-glucanase expressed in Pichia pastoris. Biochim. Biophys. Acta 1425, 419-424.
91. Hansen, O.C. and Stougaard, P. (1997) Hexose oxidase from the red alga Chondrus crispus. Purification, molecular cloning, and expression in Pichia pastoris. J. Biol. Chem. 272, 11581-11587.
92. Bojsen, K., Yu, S., Kragh, K.M. and Marcussen, J. (1999) A group of alpha-1,4-glucan lyases and their genes from the red alga Gracilariopsis lemaneiformis: purification, cloning, and heterologous expression. Biochim. Biophys. Acta 1430, 396-402.
93. Morgan, W.D., Birdsall, B., Frenkiel, T.A., Gradwell, M.G., Burghaus, P.A., Syed, S.E., Uthaipibull, C., Holder, A.A. and Feeney, J. (1999) Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1. J. Mol. Biol. 289, 113-122.
94. Kocken, C.H.M., Dubbeld, M.A., Van Der Wel, A., Pronk, J.T., Waters, A.P., Langermans, J.A.M. and Thomas, A.W. (1999) High-level expression of Plasmodium vivax apical membrane antigen 1 (AMA-1) in Pichia pastoris: strong immunogenicity in Macaca mulatta immunized with P. vivax AMA-1 and adjuvant SBAS2. Infect. Immun. 67, 43-49.
95. Linder, S., Schliwa, M. and Kube-Granderath, E. (1997) Expression of Reticulomyxa filosa tubulins in Pichia pastoris: regulation of tubulin pools. FEBS Lett. 417, 33ö37.
96. Vandersall-Nairn, A.S., Merkle, R.K., O'Brien, K., Oeltmann, T.N. and Moremen, K.W. (1998) Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi. Glycobiology 8, 1183-1194.
97. Weik, R., Francky, A., Striedner, G., Raspor, P., Bayer, K. and Mattanovich, D. (1998) Recombinant expression of alliin lyase from garlic (Allium sativum) in bacteria and yeasts. Planta Med. 64, 387-388.
98. Su, W., Mertens, J.A., Kanamaru, K., Campbell, W.H. and Crawford, N.M. (1997) Analysis of wild-type and mutant plant nitrate reductase expressed in the methylotrophic yeast Pichia pastoris. Plant Physiol. 115, 1135-1143.
99. Su, W., Huber, S.C. and Crawford, N.M. (1996) Identification in vitro of a post-translational regulatory site in the hinge 1 region of Arabidopsis nitrate reductase. Plant Cell 8, 519-527.
100. Hochstrasser, U., Luscher, M., De Virgilio, C., Boler, T. and Wiemken, A. (1998) Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris. FEBS Lett. 440, 356-360.
101. Juge, N., Andersen, J.S., Tull, D., Roepstorff, P. and Svensson, B. (1996) Overexpression, purification, and characterization of recombinant barley alpha-amylases 1 and 2 secreted by the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 8, 204-214.
102. Tibbot, B.K., Henson, C.A. and Skadsen, R.W. (1998) Expression of enzymatically active, recombinant barley alpha-glucosidase in yeast and immunological detection of alpha-glucosidase from seed tissue. Plant Mol. Biol. 38, 379-391.
103. Zhu, A., Monahan, C., Zhang, Z., Hurst, R., Leng, L. and Goldstein, J. (1995) High-level expression and purification of coffee bean alpha-galactosidase produced in the yeast Pichia pastoris. Arch. Biochem. Biophys. 324, 65-70.
104. White, P.C., Cordeiro, M.C., Arnold, D., Brodelius, P.E. and Kay, J. (1999) Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cynara cardunculus). J. Biol. Chem. 274, 16685-16693.
105. Chang, Z.N., Peng, H.J., Lee, W.C., Chen, T.S., Chua, K.Y., Tsai, L.C., Chi, C.W. and Han, S.H. (1999) Sequence polymorphism of the group 1 allergen of Bermuda grass pollen. Clin. Exp. Allergy 29, 488-496.
106. Smith, P.M., Suphioglu, C., Griffith, I.J., Theriault, K., Knox, R.B. and Singh, M.B. (1996) Cloning and expression in yeast Pichia pastoris of a biologically active form of Cyn d 1, the major allergen of Bermuda grass pollen. J. Allergy Clin. Immunol. 98, 331-343.
108. Hasslacher, M., Schall, M., Hayn, M., Bona, R., Rumbold, K., Luckl, J., Griengl, H., Kohlwein, S.D. and Schwab, H. (1997) High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial hosts. Protein Expr. Purif. 11, 61-71.
109. Sowka, S., Wagner, S., Krebitz, M., Arija-Mad-Arif, S., Yusof, F., Kinaciyan, T., Brehler, R., Scheiner, O. and Breiteneder, H. (1998) cDNA cloning of the 43-kDa latex allergen Hev b 7 with sequence similarity to patatins and its expression in the yeast Pichia pastoris. Eur. J. Biochem. 255, 213-219.
110. Breiteneder, H., Sowka, S., Wagner, S., Krebitz, M., Hafner, C., Kinaciyan, T., Yeang, H.Y. and Scheiner, O. (1999) Cloning of the patatin-like latex allergen Hev b 7, its expression in the yeast Pichia pastoris and its immunological characterization. Int. Arch. Allergy Immunol. 118, 309-310.
111. Morris, R.O., Bilyeu, K.D., Laskey, J.G. and Cheikh, N.N. (1999) Isolation of a gene encoding a glycosylated cytokinin oxidase from maize. Biochem. Biophys. Res. Commun. 255, 328-333.
112. Mozley, D., Remberg, A. and Gartner, W. (1997) Large-scale generation of affinity-purified recombinant phytochrome chromopeptide. Photochem. Photobiol. 66, 710-715.
113. Kneip, C., Mozley, D., Hildebrandt, M.P., Gartner, W., Braslavsky, S.E. and Schaffner, K. (1997) Effect of chromophore exchange on the resonance Raman spectra of recombinant phytochromes. FEBS Lett. 414, 23-26.
114. Remberg, A., Ruddat, A., Braslavsky, S.E., Gartner, W. and Schaffner, K. (1998) Chromophore incorporation, Pr to Pfr kinetics, and Pfr thermal reversion of recombinant N-terminal fragments of phytochrome A and B chromoproteins. Biochemistry 37, 9983-9990.
115. Huecas, S., Villalba, M., Gonzalez, E., Martinez-Ruiz, A. and Rodriguez, R. (1999) Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris. Eur. J. Biochem. 261, 539-546.
116. Ferrarese, L., Trainotti, L., Gattolin, S. and Casadoro, G. (1998) Secretion, purification and activity of two recombinant pepper endo-beta-1,4-glucanases expressed in the yeast Pichia pastoris. FEBS Lett. 422, 23-26.
117. Sowka, S., Hsieh, L.S., Krebitz, M., Akasawa, A., Martin, B.M., Starrett, D., Peterbauer, C.K., Scheiner, O. and Breiteneder, H. (1998) Identification and cloning of prs a 1, a 32-kDA endochitinase and major allergen of avocado, and its expression in the yeast Pichia pastoris. J. Biol. Chem. 273, 28091-28097.
118. Ruddat, A., Schmidt, P., Gatz, C., Braslavsky, S.E., Gartner, W. and Schaffner, K. (1997) Recombinant type A and B phytochromes from potato. Transient absorption spectroscopy. Biochemistry 36, 103-111.
119. Hiller, K.M., Lubahn, B.C. and Klapper, D.G. (1998) Cloning and expression of ragweed allergen Amb a 6. Scand. J. Immunol. 48, 26-36.
120. Penheiter, A.R., Klucas, R.V. and Sarath, G. (1998) Purification and characterization of a soybean root nodule phosphatase expressed in Pichia pastoris. Protein Expr. Purif. 14, 125-130.
121. Payne, M.S., Petrillo, K.L., Gavagan, J.E., Wagner, L.W., DiCosimo, R. and Anton, D.L. (1995) High-level production of spinach glycolate oxidase in the methylotrophic yeast Pichia pastoris: engineering a biocatalyst. Gene 167, 215-219.
122. Payne, M.S., Petrillo, K.L., Gavagan, J.E., DiCosimo, R., Wagner, L.W. and Anton, D.L. (1997) Engineering Pichia pastoris for biocatalysis: co-production of two active enzymes. Gene 194, 179-182.
123. Brandes, H.K., Hartman, F.C., Lu, T.Y. and Larimer, F.W. (1996) Efficient expression of the gene for spinach phosphoribulokinase in Pichia pastoris and utilization of the recombinant enzyme to explore the role of regulatory cysteinyl residues by site-directed mutagenesis. J. Biol. Chem. 271, 6490-6496.
124. Petersen, A., Grobe, K., Lindner, B., Schlaak, M. and Becker, W.M. (1997) Comparison of natural and recombinant isoforms of grass pollen allergens. Electrophoresis 18, 819-825.
125. Brenner, E.D., Lambert, K.N., Kaloshian, I. and Williamson, V.M. (1998) Characterization of LeMir, a root-knot nematode-induced gene in tomato with an encoded product secreted from the root. Plant Physiol. 118, 237-247.
126. Klein, C., de Lamotte-Guery, F., Gautier, F., Moulin, G., Boze, H., Joudrier, P. and Gautier, M.F. (1998) High-level secretion of a wheat lipid transfer protein in Pichia pastoris. Protein Expr. Purif. 13, 73-82.
127. Ogawa, M., Nakamura, S., Atsuchi, T., Tamiya, T., Tsuchiya, T. and Nakai, S. (1999) Macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast Pichia pastoris. FEBS Lett. 448, 41-44.
128. Munshi, C. and Lee, H.C. (1997) High-level expression of recombinant Aplysia ADP-ribosyl cyclase in Pichia pastoris by fermentation. Protein Expr. Purif. 11, 104-110.
129. Monosov, E.Z., Wenzel, T.J., Luers, G.H., Heyman, J.A. and Subramani, S. (1996) Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells. J. Histochem. Cytochem. 44, 581-589.
130. Gallet, P.F., Vaujour, H., Petit, J.M., Maftah, A., Oulmouden, A., Oriol, R., Le Narvor, C., Guilloton, M. and Julien, R. (1998) Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris. Glycobiology 8, 919-925.
131. Garcia-Garcia, J.C., Soto, A., Nigro, F., Mazza, M., Joglar, M., Hechevarria, M., Lamberti, J. and de la Fuente, J. (1998) Adjuvant and immunostimulating properties of the recombinant Bm86 protein expressed in Pichia pastoris. Vaccine 16, 1053-1055.
132. Garcia-Garcia, J.C., Montero, C., Rodriquez, M., Soto, A., Redondo, M., Valdes, M., Mendez, L. and de la Fuente, J. (1998) Effect of particulation on the immunogenic and protective properties of the recombinant Bm86 antigen expressed in Pichia pastoris. Vaccine 16, 374-380.
133. Rodríguez, M., Rubiera, R., Penichet, M., Montesinos, R., Cremata, J., Falcón, V., Sánchez, G., Bringas, R., Cordovés, C., Valdés, M., Lleonart, R., Herrera, L., and de la Fuente, J. (1994) High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle. J. Biotechnol. 33, 135-146.
134. Vailes, L.D., Kinter, M.T., Arruda, L.K. and Chapman, M.D. (1998) High-level expression of cockroach allergen, Bla g 4, in Pichia pastoris. J. Allergy Clin. Immunol. 101, 274-280.
135. Williams, T.A., Michaud, A., Houard, X., Chauvet, M.T., Soubrier, F. and Corvol, P. (1996) Drosophila melanogaster angiotensin I-converting enzyme expressed in Pichia pastoris resembles the C domain of the mammalian homologue and does not require glycosylation for secretion and enzymic activity. Biochem. J. 318, 125-131.
136. McCollum, D., Monosov, E. and Subramani, S. (1993) The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome÷the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family. J. Cell Biol. 121, 761-774.
137. Canales, M., Enriquez, A., Ramos, E., Cabrera, D., Dandie, H., Soto, A., Falcon, V., Rodriguez, M. and de la Fuente, J. (1997) Large-scale production in Pichia pastoris of the recombinant vaccine Gavac against cattle tick. Vaccine 15, 414-422.
138. Brankamp, R.G., Sreekrishna, K., Smith, P.L., Blankenship, D.T. and Cardin, A.D. (1995) Expression of a synthetic gene encoding the anticoagulant-antimetastatic protein ghilanten by the methylotropic yeast Pichia pastoris. Protein Expr. Purif. 6, 813-820.
139. Rosenfeld, S.A., Nadeau, D., Tirado, J., Hollis, G.F., Knabb, R.M. and Jia, S. (1996) Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 8, 476-482.
140. Briand, L., Perez, V., Huet, J.C., Danty, E., Masson, C. and Pernollet, J.C. (1999) Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris. Protein Expr. Purif. 15, 362-369.
141. Hussein, A.S., Chacon, M.R., Smith, A.M., Tosado-Acevedo, R. and Selkirk, M.E. (1999) Cloning, expression, and properties of a nonneuronal secreted acetylcholinesterase from the parasitic nematode Nippostrongylus brasiliensis. J. Biol. Chem. 274, 9312-9319.
142. Fahnestock, S.R. and Bedzyk, L.A. (1997) Production of synthetic spider dragline silk protein in Pichia pastoris. Appl. Microbiol. Biotechnol. 47, 33ö39.
143. Laroche, Y., Storme, V., De Meutter, J., Messens, J. and Lauwereys, M. (1994) High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris. Biotechnology (NY) 12, 1119-1124.
144. Vozza, L.A., Wittwer, L., Higgins, D.R., Purcell, T.J., Bergseid, M., Collins-Racie, L.A., LaVallie, E.R. and Hoeffler, J.P. (1996) Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris. Biotechnology (NY) 14, 77-81.
145. Samaddar, M., Catterall, J.F. and Dighe, R.R. (1997) Expression of biologically active beta subunit of bovine follicle-stimulating hormone in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 10, 345-355.
146. Rodriguez, M., Martinez, V., Alazo, K., Suarez, M., Redondo, M., Montero, C., Besada, V. and de la Fuente, J. (1998) The bovine IFN-omega 1 is biologically active and secreted at high levels in the yeast Pichia pastoris. J. Biotechnol. 60, 3-14.
147. Digan, M.E., Lair, S.V., Brierley, R.A., Siegel, R.S., Williams, M.E., Ellis, S.B., Kellaris, P.A., Provow, S.A., Craig, W.S., Veliçelebi, G., Harpold, M.M. and Thill, G.P. (1989) Continuous production of a novel lysozyme via secretion from the yeast, Pichia pastoris. Bio/Technology 7, 160-164.
148. Abdulaev, N.G., Popp, M.P., Smith, W.C. and Ridge, K.D. (1997) Functional expression of bovine opsin in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 10, 61-69.
149. Vedvick, T., Buckholtz, R.G., Engel, M., Urcan, M., Kinney, J., Provow, S., Siegel, R.S. and Thill, G.P. (1991) High-level secretion of biologically active aprotinin from the yeast Pichia pastoris. J. Ind. Microbiol. 7, 197-201.
150. Choi, B.-K. and Jimenez-Flores, R. (1996) Study of putative glycosylation site in bovine beta-casein introduced by PCR-based site-directed mutagenesis. J. Agric. Food Chem. 44, 358-364.
151. Denton, H., Smith, M., Husi, H., Uhrin, D., Barlow, P.N., Batt, C.A. and Sawyer, L. (1998) Isotopically labeled bovine beta-lactoglobulin for NMR studies expressed in Pichia pastoris. Protein Expr. Purif 14, 97-103.
152. Kim, T.R., Goto, Y., Hirota, N., Kuwata, K., Denton, H., Wu, S.K., Sawyer, L. and Batt, C.A. (1997) High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical properties. Protein Eng. 10, 1339-1345.
153. Uhrinova, S., Uhrin, D., Denton, H., Smith, M., Sawyer, L. and Barlow, P.N. (1998) Complete assignment of 1H, 13C and 15N chemical shifts for bovine beta-lactoglobulin: secondary structure and topology of the native state is retained in a partially unfolded form. J. Biomol. NMR 12, 89-107.
154. Johnsen, L.B., Ravn, P., Berglund, L., Petersen, T.E., Rasmussen, L.K., Heegaard, C.W., Rasmussen, J.T., Benfeldt, C. and Fedosov, S.N. (1998) A refined kinetic analysis of plasminogen activation by recombinant bovine tissue-type plasminogen activator indicates two interconvertible activator forms. Biochemistry 37, 12631-12639.
155. Wedlock, D.N., Goh, L.P., McCarthy, A.R., Midwinter, R.G., Parlane, N.A. and Buddle, B.M. (1999) Physiological effects and adjuvanticity of recombinant brushtail possum TNF-alpha. Immunol. Cell Biol. 77, 28-33.
156. Morel, N. and Massoulie, J. (1997) Expression and processing of vertebrate acetylcholinesterase in the yeast Pichia pastoris. Biochem. J. 328, 121-129.
157. Zhu, A., Monahan, C., Wang, Z.K. and Goldstein, J. (1996) Expression, purification, and characterization of recombinant alpha-N-acetylgalactosaminidase produced in the yeast Pichia pastoris. Protein Expr. Purif. 8, 456-462.
158. Simon, S. and Massoulie, J. (1997) Cloning and expression of acetylcholinesterase from Electrophorus. Splicing pattern of the 3_ exons in vivo and in transfected mammalian cells. J. Biol. Chem. 272, 33045-33055.
159. Mine, S., Ueda, T., Hashimoto, Y., Tanaka, Y. and Imoto, T. (1999) High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements. FEBS Lett. 448, 33-37.
160. Rautiainen, J., Auriola, S., Rouvinen, J., Kauppinen, J., Zeiler, T., Novikov, D., Virtanen, T. and Mantyjarvi, R.A. (1998) Molecular and crystal properties of Bos d 2, an allergenic protein of the lipocalin family. Biochem. Biophys. Res. Commun. 247, 746-750.
161. Weiss, H.M., Haase, W., Michel, H. and Reilander, H. (1998) Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human beta2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris. Biochem. J. 330, 1137-1147.
162. Clare, J.J., Romanos, M.A., Rayment, F.B., Rowedder, J.E., Smith, M.A., Payne, M.M., Sreekrishna, K. and Henwood, C.A. (1991) Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205-212.
163. Masure, S., Paemen, L., Van Aelst, I., Fiten, P., Proost, P., Billiau, A., Van Damme, J. and Opdenakker, G. (1997) Production and characterization of recombinant active mouse gelatinase B from eukaryotic cells and in vivo effects after intravenous administration. Eur. J. Biochem. 244, 21-30.
164. Merkle, R.K., Zhang, Y., Ruest, P.J., Lal, A., Liao, Y.F. and Moremen, K.W. (1997) Cloning, expression, purification, and characterization of the murine lysosomal acid alpha-mannosidase. Biochim. Biophys. Acta 1336, 132-146.
165. Ferrari, E., Lodi, T., Sorbi, R.T., Tirindelli, R., Cavaggioni, A. and Spisni, A. (1997) Expression of a lipocalin in Pichia pastoris: secretion, purification and binding activity of a recombinant mouse major urinary protein. FEBS Lett. 401, 73-77.
166. Urbatsch, I.L., Beaudet, L., Carrier, I. and Gros, P. (1998) Mutations in either nucleotide-binding site of P-glycoprotein (Mdr3) prevent vanadate trapping of nucleotide at both sites. Biochemistry 37, 4592-4602.
167. Beaudet, L., Urbatsch, I.L. and Gros, P. (1998) High-level expression of mouse Mdr3 P-glycoprotein in yeast Pichia pastoris and characterization of ATPase activity. Methods Enzymol. 292, 397-413.
168. Beaudet, L., Urbatsch, I.L. and Gros, P. (1998) Mutations in the nucleotide-binding sites of P-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity. Biochemistry 37, 9073-9082.
169. Eldin, P., Pauza, M.E., Hieda, Y., Lin, G., Murtaugh, M.P., Pentel, P.R. and Pennell, C.A. (1997) High-level secretion of two antibody single chain Fv fragments by Pichia pastoris. J. Immunol. Methods 201, 67-75.
170. Boehm, T., Pirie-Shepard, S., Trinh, L.B., Shiloach, J. and Folkman, J. (1999) Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin. Yeast 15, 563-567.
171. Vallee, F., Lal, A., Moremen, K.W. and Howell, P.L. (1999) Purification, crystallization and preliminary X-ray crystallographic analysis of recombinant murine Golgi mannosidase IA, a class I alpha-mannosidase involved in Asn-linked oligosaccharide maturation. Acta Crystallogr. D Biol. Crystallogr. 55, 571-573.
172. Jerva, L.F., Sullivan, G. and Lolis, E. (1997) Functional and receptor binding characterization of recombinant murine macrophage inflammatory protein 2: sequence analysis and mutagenesis identify receptor binding epitopes. Protein Sci. 6, 1643-1652.
173. Fidler, A.E., Lun, S., Young, W. and McNatty, K.P. (1998) Expression and secretion of a biologically active glycoprotein hormone, ovine follicle stimulating hormone, by Pichia pastoris. J. Mol. Endocrinol. 21, 327-336.
174. Richard, F., Robert, P., Remy, J.J., Martinat, N., Bidart, J.M., Salesse, R. and Combarnous, Y. (1998) High-level secretion of biologically active recombinant porcine follicle-stimulating hormone by the methylotrophic yeast Pichia pastoris. Biochem. Biophys. Res. Commun. 245, 847-852.
175. Wuebbens, M.W., Roush, E.D., Decastro, C.M. and Fierke, C.A. (1997) Cloning, sequencing, and recombinant expression of the porcine inhibitor of carbonic anhydrase: a novel member of the transferrin family. Biochemistry 36, 4327-4336.
176. Reddy, R.G., Yoshimoto, T., Yamamoto, S. and Marnett, L.J. (1994) Expression, purification, and characterization of porcine leukocyte 12-lipoxygenase produced in the methylotrophic yeast, Pichia pastoris. Biochem. Biophys. Res. Commun. 205, 381-388.
177. Doring, F., Theis, S. and Daniel, H. (1997) Expression and functional characterization of the mammalian intestinal peptide transporter PepT1 in the methylotrophic yeast Pichia pastoris. Biochem. Biophys. Res. Commun. 232, 656-662.
178. Doring, F., Michel, T., Rosel, A., Nickolaus, M. and Daniel, H. (1998) Expression of the mammalian renal peptide transporter PEPT2 in the yeast Pichia pastoris and applications of the yeast system for functional analysis. Mol. Membr. Biol. 15, 79-88.
179. Ridder, R., Schmitz, R., Legay, F. and Gram, H. (1995) Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris. Biotechnology (NY) 13, 255-260.
180. Kotake, H., Li, Q., Ohnishi, T., Ko, K.W., Agellon, L.B. and Yokoyama, S. (1996) Expression and secretion of rabbit plasma cholesteryl ester transfer protein by Pichia pastoris. J. Lipid Res. 37, 599-605.
181. Sadhukhan, R., Sen, G.C. and Sen, I. (1996) Synthesis and cleavage÷secretion of enzymatically active rabbit angiotensin-converting enzyme in Pichia pastoris. J. Biol. Chem. 271, 18310-18313.
182. Heim, J., Schmidt-Dannert, C., Atomi, H. and Schmid, R.D. (1998) Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli. Biochim. Biophys. Acta 1396, 306-319.
183. He, C., Alexander, J.J., Lim, A. and Quigg, R.J. (1997) Production of the rat complement regulator, Crry, as an active soluble protein in Pichia pastoris. Arch. Biochem. Biophys. 341, 347-352.
184. Chen, Y.J. and Gonatas, N.K. (1997) The Golgi sialoglycoprotein MG160, expressed in Pichia pastoris, does not require complex carbohydrates and sialic acid for secretion and basic fibroblast growth factor binding. Biochem. Biophys. Res. Commun. 234, 68-72.
185. Mistry, A.R., Falciola, L., Monaco, L., Tagliabue, R., Acerbis, G., Knight, A., Harbottle, R.P., Soria, M., Bianchi, M.E., Coutelle, C. and Hart, S.L. (1997) Recombinant HMG1 protein produced in Pichia pastoris: a nonviral gene delivery agent. Biotechniques 22, 718-729.
186. Zhu, H., Shi, J., de Vries, Y., Arvidson, D.N., Cregg, J.M. and Woldegiorgis, G. (1997) Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I. Arch. Biochem. Biophys. 347, 53-61.
187. de Vries, Y., Arvidson, D.N., Waterham, H.R., Cregg, J.M. and Woldegiorgis, G. (1997) Functional characterization of mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris. Biochemistry 36, 5285-5292.
188. Gachhui, R., Presta, A., Bentley, D.F., Abu-Soud, H.M., McArthur, R., Brudvig, G., Ghosh, D.K. and Stuehr, D.J. (1996) Characterization of the reductase domain of rat neuronal nitric oxide synthase generated in the methylotrophic yeast Pichia pastoris. Calmodulin response is complete within the reductase domain itself. J. Biol. Chem. 271, 20594-20602.
189. Qin, Y.M., Poutanen, M.H., Helander, H.M., Kvist, A.P., Siivari, K.M., Schmitz, W., Conzelmann, E., Hellman, U. and Hiltunen, J.K. (1997) Peroxisomal multifunctional enzyme of beta-oxidation metabolizing D-3-hydroxyacyl-CoA esters in rat liver: molecular cloning, expression and characterization. Biochem. J. 321, 21-28.
190. Yu, Y., Vranken, W., Goudreau, N., de Miguel, E., Magny, M.C., Mort, J.S., Dupras, R., Storer, A.C. and Ni, F. (1998) An NMR-based identification of peptide fragments mimicking the interactions of the cathepsin B propeptide. FEBS Lett. 429, 9-16.
191. Sivaraman, J., Coulombe, R. and Cygler, M. (1996) Crystallization of rat procathepsin B. Acta Crystallogr. 52, 874.
191. Cygler, M., Sivaraman, J., Grochulski, P., Coulombe, R., Storer, A.C., Mort, J.S. (1996) Structure of rat procathepsin B: model for inhibition of cysteine protease activity by the proregion. Structure 4(4):405-16.
192. Gronwald, W., Loewen, M.C., Lix, B., Daugulis, A.J., Sonnichsen, F.D., Davies, P.L. and Sykes, B.D. (1998) The solution structure of type II antifreeze protein reveals a new member of the lectin family. Biochemistry 37, 4712-4721.
193. Loewen, M.C., Liu, X., Davies, P.L. and Daugulis, A.J. (1997) Biosynthetic production of type II fish antifreeze protein: fermentation by Pichia pastoris. Appl. Microbiol. Biotechnol. 48, 480-486.
194. Trant, J.M. (1996) Functional expression of recombinant spiny dogfish shark (Squalus acanthias) cytochrome P450c17 (17 alpha-hydroxylase/C17,20-lyase) in yeast (Pichia pastoris). Arch. Biochem. Biophys. 326, 8-14.
195. Weiss, S., Famulok, M., Edenhofer, F., Wang, Y.öH., Jones, I.M., Groschup, M. and Winnacker, E.L. (1995) Overexpression of active Syrian golden hamster prion protein PrPc as a glutathione S-transferase fusion in heterologous systems. J. Virol. 69, 4776-4783.
196. Tremblay, L.O., Campbell Dyke, N. and Herscovics, A. (1998) Molecular cloning, chromosomal mapping and tissue-specific expression of a novel human alpha1,2-mannosidase gene involved in N-glycan maturation. Glycobiology 8, 585-595.
197. Zhu, A., Wang, Z.K. and Beavis, R. (1998) Structural studies of alpha-N-acetylgalactosaminidase: effect of glycosylation on the level of expression, secretion efficiency, and enzyme activity. Arch. Biochem. Biophys. 352, 1-8.
198. Kang, H.A., Sohn, J.H., Choi, E.S., Chung, B.H., Yu, M.H. and Rhee, S.K. (1998) Glycosylation of human alpha 1-antitrypsin in Saccharomyces cerevisiae and methylotrophic yeasts. Yeast 14, 371-381.
199. Talmont, F., Sidobre, S., Demange, P., Milon, A. and Emorine, L.J. (1996) Expression and pharmacological characterization of the human mu-opoid receptor in the methylotrophic yeast Pichia pastoris. FEBS Lett. 394, 268-272.
200. O'Connell, M.A., Gerber, A. and Keegan, L.P. (1998) Purification of native and recombinant double-stranded RNA-specific adenosine deaminases. Methods 15, 51-62.
201. Le Brocque, D., Henry, A., Cappai, R., Li, Q.X., Tanner, J.E., Galatis, D., Gray, C., Holmes, S., Underwood, J.R., Beyreuther, K., Masters, C.L. and Evin, G. (1998) Processing of the Alzheimer's disease amyloid precursor protein in Pichia pastoris: immunodetection of alpha-, beta-, and gamma-secretase products. Biochemistry 37, 14958-14965.
202. Mok, S.S., Sberna, G., Heffernan, D., Cappai, R., Galatis, D., Clarris, H.J., Sawyer, W.H., Beyreuther, K., Masters, C.L. and Small, D.H. (1997) Expression and analysis of heparin-binding regions of the amyloid precursor protein of Alzheimer's disease. FEBS Lett. 415, 303-307.
203. Cappai, R., Mok, S.S., Galatis, D., Tucker, D.F., Henry, A., Beyreuther, K., Small, D.H. and Masters, C.L. (1999) Recombinant human amyloid precursor-like protein 2 (APLP2) expressed in the yeast Pichia pastoris can stimulate neurite outgrowth. FEBS Lett. 442, 95-98.
204. Henry, A., Masters, C.L., Beyreuther, K. and Cappai, R. (1997) Expression of human amyloid precursor protein ectodomains in Pichia pastoris: analysis of culture conditions, purification, and characterization. Protein Expr. Purif. 10, 283-291.
205. Culvenor, J.G., Henry, A., Hartmann, T., Evin, G., Galatis, D., Friedhuber, A., Jayasena, U.L., Underwood, J.R., Beyreuther, K., Masters, C.L. and Cappai, R. (1998) Subcellular localization of the Alzheimer's disease amyloid precursor protein and derived polypeptides expressed in a recombinant yeast system. Amyloid 5, 79-89.
206. Ohsawa, I., Hirose, Y., Ishiguro, M., Imai, Y., Ishiura, S. and Kohsaka, S. (1995) Expression, purification, and neurotrophic activity of amyloid precursor protein-secreted forms produced by yeast. Biochem. Biophys. Res. Commun. 213, 52-58.
207. Sahasrabudhe, A.V., Solapure, S.M., Khurana, R., Suryanarayan, V., Ravishankar, S., deSousa, S.M. and Das, G. (1998) Production of recombinant human bile salt stimulated lipase and its variant in Pichia pastoris. Protein Expr. Purif. 14, 425-433.
208. Fitzgerald, K., Hollinger, P. and Winter, G. (1997) Improved tumour targeting by disulphide stabilized diabodies expressed in Pichia pastoris. Protein Eng. 10, 1221ö1225.
209. Lam, L.P. and Berger, S.A. (1997) Intracellular expression and purification of the cökit receptor kinase domain in Pichia pastoris. Biotechniques 23, 83-86.
210. Lam, L.P., Chow, R.Y. and Berger, S.A. (1999) A transforming mutation enhances the activity of the c-Kit soluble tyrosine kinase domain. Biochem. J. 338, 131-138.
211. You, Y.H., Hefta, L.J., Yazaki, P.J., Wu, A.M. and Shively, J.E. (1998) Expression, purification, and characterization of a two domain carcinoembryonic antigen minigene (N-A3) in Pichia pastoris. The essential role of the N-domain. Anticancer Res. 18, 3193-3201.
212. Sun, J., Bottomley, S.P., Kumar, S. and Bird, P.I. (1997) Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme. Biochem. Biophys. Res. Commun. 238, 920-924.
213. Linnevers, C.J., McGrath, M.E., Armstrong, R., Mistry, F.R., Barnes, M.G., Klaus, J.L., Palmer, J.T., Katz, B.A. and Bromme, D. (1997) Expression of human cathepsin K in Pichia pastoris and preliminary crystallographic studies of an inhibitor complex. Protein Sci. 6, 919-921.
214. Hou, W.S., Brommer, D., Zhao, Y., Mehler, E., Dushey, C., Weinstein, H., Miranda, C.S., Fraga, C., Greig, F., Carey, J., Rimoin, D.L., Desnick, R.J. and Gelb, B.D. (1999) Characterization of novel cathepsin K mutations in the pro and mature polypeptide regions causing pycnodysostosis. J. Clin. Invest. 103, 731-738.
215. Menard, R., Carmona, E., Takebe, S., Dufour, E., Plouffe, C., Mason, P. and Mort, J.S. (1998) Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro. J. Biol. Chem. 273, 4478-4484.
216. Carmona, E., Dufour, E., Plouffe, C., Takebe, S., Mason, P., Mort, J.S. and Menard, R. (1996) Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases. Biochemistry 35, 8149-8157.
217. Bromme, D., Li, Z., Barnes, M. and Mehler, E. (1999) Human cathepsin V functional expression, tissue distribution, electrostatic surface potential, enzymatic characterization, and chromosomal localization. Biochemistry 38, 2377-2385.
218. Munshi, C.B., Fryxell, K.B., Lee, H.C. and Branton, W.D. (1997) Large-scale production of human CD38 in yeast by fermentation. Methods Enzymol. 280, 318-330.
219. McGrew, J.T., Leiske, D., Dell, B., Klinke, R., Krasts, D., Wee, S.F., Abbott, N., Armitage, R. and Harrington, K. (1997) Expression of trimeric CD40 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants. Gene 187, 193-200.
220. Gerstmayer, B., Altenschmidt, U., Hoffmann, M. and Wels, W. (1997) Costimulation of T cell proliferation by a chimeric B7-2 antibody fusion protein specifically targeted to cells expressing the erbB2 proto-oncogene. J. Immunol. 158, 4584-4590.
221. Sen Gupta, C. and Dighe, R.R. (1999) Hyperexpression of biologically active human chorionic gonadotropin using the methylotropic yeast Pichia pastoris. J. Mol. Endocrinol. 22, 273-283.
222. Daniels, G.L., Green, C.A., Powell, R.M. and Ward, T. (1998) Hemagglutination inhibition of Cromer blood group antibodies with soluble recombinant decay-accelerating factor. Transfusion 38, 332-336.
223. Battista, M.C., Bergamini, G., Campanini, F., Landini, M.P. and Ripalti, A. (1996) Intracellular production of a major cytomegalovirus antigenic protein in the methylotrophic yeast Pichia pastoris. Gene 176, 197-201.
224. Powell, R.M., Ward, T., Evans, D.J. and Almond, J.W. (1997) Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): evidence for a secondary cellular factor in A-particle formation. J. Virol. 71, 9306-9312.
225. Gerber, A., O'Connell, M.A. and Keller, W. (1997) Two forms of human double-stranded RNA-specific editase 1 (hRED1) generated by the insertion of an Alu cassette. RNA 3, 453-463.
226. Boehm, T., O'Reilly, M.S., Keough, K., Shiloach, J., Shapiro, R. and Folkman, J. (1998) Zinc-binding of endostatin is essential for its angiogenic activity. Biochem. Biophys. Res. Commun. 252, 190-194.
227. Tanaka, M., Suda, T., Yatomi, T., Nakamura, N. and Nagata, S. (1997) Lethal effect of recombinant human Fas ligand in mice pretreated with Propionibacterium acnes. J. Immunol. 158, 2303-2309.
228. Cote, H.C., Pratt, K.P., Davie, E.W. and Chung, D.W. (1997) The polymerization pocket "a" within the carboxy-terminal region of the gamma chain of human fibrinogen is adjacent to but independent from the calcium-binding site. J. Biol. Chem. 272, 23792-23798.
229. Rosenfeld, S.A., Ross, O.H., Hillman, M.C., Corman, J.I. and Dowling, R.L. (1996) Production and purification of human fibroblast collagenase (MMP-1) expressed in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 7, 423-430.
230. Spraggon, G., Applegate, D., Everse, S.J., Zhang, J.Z., Veerapandian, L., Redman, C., Doolittle, R.F. and Grieninger, G. (1998) Crystal structure of a recombinant alphaEC domain from human fibrinogen-420. Proc. Natl. Acad. Sci. USA 95, 9099-9104.
231. Yamada, M., Azuma, T., Matsuba, T., Iida, H., Suzuki, H., Yamamoto, K., Kohli, Y. and Hori, H. (1994) Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methylotrophic yeast, Pichia pastoris and characterization of produced recombinant cathepsin E. Biochim. Biophys. Acta 1206, 279-285.
232. Zhu, H., Shi, J., Cregg, J.M. and Woldegiorgis, G. (1997) Reconstitution of highly expressed human heart muscle carnitine palmitoyltransferase I. Biochem. Biophys. Res. Commun. 239, 498-502.
233. Kjeldsen, T., Pettersson, A.F. and Hach, M. (1999) Secretory expression and characterization of insulin in Pichia pastoris. Biotechnol. Appl. Biochem. 29, 79ö86.
234. Brierley, R.A. (1998) Secretion of recombinant human insulin-like growth factor I (IGF-1). Methods Mol. Biol. 103, 149-177.
235. Green, M.M., Larkin, J., 3rd, Subramaniam, P.S., Szente, B.E. and Johnson, H.M. (1998) Human IFN gamma receptor cytoplasmic domain: expression and interaction with HuIFN gamma. Biochem. Biophys. Res. Commun. 243, 170-176.
236. Murphy, K.P., Jr., Gagne, P., Pazmany, C. and Moody, M.D. (1998) Expression of human interleukin-17 in Pichia pastoris: purification and characterization. Protein Expr. Purif. 12, 208-214.
237. Sun, J., Coughlin, P., Salem, H.H. and Bird, P. (1995) Production and characterization of recombinant human proteinase inhibitor 6 expressed in Pichia pastoris. Biochim. Biophys. Acta 1252, 28-34.
238. Wagner, S.L., Siegel, R.S., Vedvick, T.S., Raschke, W.C. and Van Nostrand, W.E. (1992) High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid beta-protein precursor. Biochem. Biophys. Res. Commun. 186, 1138-1145.
239. Zhang, J.G., Owczarek, C.M., Ward, L.D., Howlett, G.J., Fabri, L.J., Roberts, B.A. and Nicola, N.A. (1997) Evidence for the formation of a heterotrimeric complex of leukaemia inhibitory factor with its receptor subunits in solution. Biochem. J. 325, 693-700.
240. Fryxell, K.B., O'Donoghue, K., Graeff, R.M., Lee, H.C. and Branton, W.D. (1995) Functional expression of soluble forms of human CD38 in Escherichia coli and Pichia pastoris. Protein Expr. Purif. 6, 329-336.
241. Liao, Y.F., Lal, A. and Moremen, K.W. (1996) Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid alpha-mannosidase. J. Biol. Chem. 271, 28348-28358.
242. Chan, H., Elrod, K.C., Numerof, R.P., Sideris, S. and Clark, J.M. (1999) Expression and characterization of recombinant mast cell tryptase. Protein Expr. Purif. 15, 251-257.
243. Niles, A.L., Maffitt, M., Haak-Frendscho, M., Wheeless, C.J. and Johnson, D.A. (1998) Recombinant human mast cell tryptase beta: stable expression in Pichia pastoris and purification of fully active enzyme. Biotechnol. Appl. Biochem. 28, 125-131.
244. Kalandadze, A., Galleno, M., Foncerrada, L., Strominger, J.L. and Wucherpfennig, K.W. (1996) Expression of recombinant HLA-DR2 molecules. Replacement of the hydrophobic transmembrane region by a leucine zipper dimerization motif allows the assembly and secretion of soluble DR alpha beta heterodimers. J. Biol. Chem. 271, 20156-20162.
245. Luo, D., Mah, N., Krantz, M., Wilde, K., Wishart, D., Zhang, Y., Jacobs, F. and Martin, L. (1995) Vl-linker-Vh orientation-dependent expression of single chain Fv-containing an engineered disulfide-stabilized bond in the framework regions. J. Biochem. (Tokyo) 118, 825-831.
246. Beall, C.J., Breckenridge, S.M., Chakravarty, L. and Kolattukudy, P.E. (1998) Expression of human monocyte chemoattractant protein-1 in the yeast Pichia pastoris. Protein Expr. Purif. 12, 145-150.
247. Masure, S., Paemen, L., Proost, P., Van Damme, J. and Opdenakker, G. (1995) Expression of a human mutant monocyte chemotactic protein 3 in Pichia pastoris and characterization as an MCP-3 receptor antagonist. J. Interferon Cytokine Res. 15, 955-963.
248. Kiselyov, V.V., Berezin, V., Maar, T.E., Soroka, V., Edvardsen, K., Schousboe, A. and Bock, E. (1997) The first immunoglobulin-like neural cell adhesion molecule (NCAM) domain is involved in double-reciprocal interaction with the second immunoglobulin-like NCAM domain and in heparin binding. J. Biol. Chem. 272, 10125-10134.
249. Yang, Y.S., Yang, M.C., Tucker, P.W. and Capra, J.D. (1997) NonO enhances the association of many DNA-binding proteins to their targets. Nucleic Acids Res. 25, 2284-2292.
250. Rydberg, E.H., Sidhu, G., Vo, H.C., Hewitt, J., Cote, H.C., Wang, Y., Numao, S., MacGillivray, R.T., Overall, C.M., Brayer, G.D. and Withers, S.G. (1999) Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris. Protein Sci. 8, 635-643.
251. Yang, Y. and Lowe, M.E. (1998) Human pancreatic triglyceride lipase expressed in yeast cells: purification and characterization. Protein Expr. Purif. 13, 36-40.
252. Dufour, E., Tam, W., Nagler, D.K., Storer, A.C. and Menard, R. (1998) Synthesis of amidrazones using an engineered papain nitrile hydratase. FEBS Lett. 433, 78-82.
253. Heimo, H., Palmu, K. and Suominen, I. (1998) Human placental alkaline phosphatase: expression in Pichia pastoris, purification and characterization of the enzyme. Protein Expr. Purif. 12, 85-92.
254. Dutta, B., Mukhopadhyay, D., Roy, N., Das, G. and Karande, A.A. (1998) Cloning, expression, purification, and immunocharacterization of placental protein-14. Protein Expr. Purif. 14, 327-334.
255. Duman, J.G., Miele, R.G., Liang, H., Grella, D.K., Sim, K.L., Castellino, F.J. and Bretthauer, R.K. (1998) O-Mannosylation of Pichia pastoris cellular and recombinant proteins. Biotechnol. Appl. Biochem. 28, 39-45.
256. Sim, B.K., O'Reilly, M.S., Liang, H., Fortier, A.H., He, W., Madsen, J.W., Lapcevich, R. and Nacy, C.A. (1997) A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer. Cancer Res. 57, 1329-1334.
257. Reverter, D., Ventura, S., Villegas, V., Vendrell, J. and Aviles, F.X. (1998) Overexpression of human procarboxypeptidase A2 in Pichia pastoris and detailed characterization of its activation pathway. J. Biol. Chem. 273, 3535-3541.
258. Illy, C., Quraishi, O., Wang, J., Purisima, E., Vernet, T. and Mort, J.S. (1997) Role of occluding loop in cathepsin B activity. J. Biol. Chem. 272, 1197-1202.
259. Cordle, R.A. and Lowe, M.E. (1998) Purification and characterization of human procolipase expressed in yeast cells. Protein Expr. Purif. 13, 30-35.
260. Lima, C.D., Klein, M.G., Weinstein, I.B. and Hendrickson, W.A. (1996) Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins. Proc. Natl. Acad. Sci. USA 93, 5357-5362.
261. Harmsen, M.C., Heeringa, P., van der Geld, Y.M., Huitema, M.G., Klimp, A., Tiran, A. and Kallenberg, C.G. (1997) Recombinant proteinase 3 (Wegener's antigen) expressed in Pichia pastoris is functionally active and is recognized by patient sera. Clin. Exp. Immunol. 110, 257-264.
262. Dahlen, J.R., Foster, D.C. and Kisiel, W. (1997) Expression, purification, and inhibitory properties of human proteinase inhibitor. Biochemistry 36, 14874-14882.
263. Luo, D., Geng, M., Schultes, B., Ma, J., Xu, D.Z., Hamza, N., Qi, W., Noujaim, A.A. and Madiyalakan, R. (1998) Expression of a fusion protein of scFv-biotin mimetic peptide for immunoassay. J. Biotechnol. 65, 225-228.
264. Pennell, C.A. and Eldin, P. (1998) In vitro production of recombinant antibody fragments in Pichia pastoris. Res. Immunol. 149, 599-603.
265. Ohtani, W., Nawa, Y., Takeshima, K., Kamuro, H., Kobayashi, K. and Ohmura, T. (1998) Physicochemical and immunochemical properties of recombinant human serum albumin from Pichia pastoris. Anal. Biochem. 256, 56-62.
266. Barr, K.A., Hopkins, S.A. and Sreekrishna, K. (1992) Protocol for efficient secretion of HSA developed from Pichia pastoris. Pharm. Eng. 12, 48-51.
267. Ikegaya, K., Hirose, M., Ohmura, T. and Nokihara, K. (1997) Complete determination of disulfide forms of purified recombinant human serum albumin, secreted by the yeast Pichia pastoris. Anal. Chem. 69, 1986-1991.
268. Ohtani, W., Ohda, T., Sumi, A., Kobayashi, K. and Ohmura, T. (1998) Analysis of Pichia pastoris components in recombinant human serum albumin by immunological assays and by HPLC with pulsed amperometric detection. Anal. Chem. 70, 425-429.
269. Steinlein, L.M., Graf, T.N. and Ikeda, R.A. (1995) Production and purification of N-terminal half-transferrin in Pichia pastoris. Protein Expr. Purif. 6, 619-624.
270. Bewley, M.C., Tam, B.M., Grewal, J., He, S., Shewry, S., Murphy, M.E., Mason, A.B., Woodworth, R.C., Baker, E.N. and MacGillivray, R.T. (1999) X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32. Biochemistry 38, 2535-2541.
271. Mason, A.B., Woodworth, R.C., Oliver, R.W.A., Green, B.N., Lin, L.N., Brandts, J.F., Tam, B.M., Maxwell, A. and MacGillivray, R.T. (1996) Production and isolation of the recombinant N-lobe of human serum transferrin from the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 8, 119-125.
272. Sui, L.M., Lennon, J., Ma, C., McCann, I., Woo, I. and Petra, P.H. (1999) Heterologous expression of wild type and deglycosylated human sex steroid-binding protein (SBP or SHBG) in the yeast, Pichia pastoris. Characterization of the recombinant proteins. J. Steroid Biochem. Mol. Biol. 68, 119-127.
273. Tsujikawa, M., Okabayashi, K., Morita, M. and Tanabe, T. (1996) Secretion of a variant of human single-chain urokinase-type plasminogen activator without an N-glycosylation site in the methylotrophic yeast, Pichia pastoris and characterization of the secreted product. Yeast 12, 541-553.
274. White, C.E., Hunter, M.J., Meininger, D.P., White, L.R. and Komives, E.A. (1995) Large-scale expression, purification and characterization of small fragments of thrombomodulin: the roles of the sixth domain and of methionine 388. Protein Eng. 8, 1177-1187.
275. Austin, A.J., Jones, C.E. and Heeke, G.V. (1998) Production of human tissue factor using the Pichia pastoris expression system. Protein Expr. Purif. 13, 136ö142.
276. Chan, H., Springmann, E.B. and Clark, J.M. (1998) Expression and characterization of human tissue kallikrein variants. Protein Expr. Purif. 12, 361-370.
277. Katz, B.A., Liu, B., Barnes, M. and Springman, E.B. (1998) Crystal structure of recombinant human tissue kallikrein at 2.0 A resolution. Protein Sci. 7, 875-885.
278. Cregg, J.M., Madden, K.R., Barringer, K.J., Thill, G.P. and Stillman, C.A. (1989) Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris. Mol. Cell. Biol. 9, 1316-1323.
279. Cregg, J.M. and Madden, K.R. (1989) Use of site-specific recombination to regenerate selectable markers. Mol. Gen. Genet. 219, 320-323.
280. Miele, R.G., Castellino, F.J. and Bretthauer, R.K. (1997) Characterization of the acidic oligosaccharides assembled on the Pichia pastoris-expressed recombinant kringle 2 domain of human tissue-type plasminogen activator. Biotechnol. Appl. Biochem. 26, 79-83.
281. Miele, R.G., Nilsen, S.L., Brito, T., Bretthauer, R.K. and Castellino, F.J. (1997) Glycosylation properties of the Pichia pastoris-expressed recombinant kringle 2 domain of tissue-type plasminogen activator. Biotechnol. Appl. Biochem. 25, 151-157.
282. Nilsen, S.L., DeFord, M.E., Prorok, M., Chibber, B.A., Bretthauer, R.K. and Castellino, F.J. (1997) High-level secretion in Pichia pastoris and biochemical characterization of the recombinant kringle 2 domain of tissue-type plasminogen activator. Biotechnol. Appl. Biochem. 25, 63-74.
283. Chang, Y., Zajicek, J. and Castellino, F.J. (1997) Role of tryptophan-63 of the kringle 2 domain of tissue-type plasminogen activator in its thermal stability, folding, and ligand binding properties. Biochemistry 36, 7652-7663.
284. Glansbeek, H.L., van Beuningen, H.M., Vitters, E.L., van der Kraan, P.M. and van den Berg, W.B. (1998) Expression of recombinant human soluble type II transforming growth factor-beta receptor in Pichia pastoris and Escherichia coli: two powerful systems to express a potent inhibitor of transforming growth factor-beta. Protein Expr. Purif. 12, 201-207.
285. Sreekrishna, K., Potenz, R.H.B., Cruze, J.A., McCombie, W.R., Parker, K.A., Nelles, L., Mazzaferro, P.K., Holden, K.A., Harrison, R.G., Wood, P.J., Phelps, D.A., Hubbard, C.E. and Fuke, M. (1988) High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris. J. Basic Microbiol. 28, 265-278.
286. Sreekrishna, K., Nelles, L., Potenz, R., Cruze, J., Mazzaferro, P., Fish, W., Fuke, M., Holden, K., Phelps, D., Wood, P. and Parker, K. (1989) High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry 28, 4117-4125.
287. Rodenburg, K.W., Kjoller, L., Petersen, H.H. and Andreasen, P.A. (1998) Binding of urokinase-type plasminogen activator-plasminogen activator inhibitor-1 complex to the endocytosis receptors alpha2-macroglobulin receptor/low-density lipoprotein receptor-related protein and very-low-density lipoprotein receptor involves basic residues in the inhibitor. Biochem. J. 329, 55-63.
288. Vuorela, A., Myllyharju, J., Nissi, R., Pihlajaniemi, T. and Kivirikko, K.I. (1997) Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast Pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase. EMBO J. 16, 6702-6712.
289. Okabayashi, K., Tsujikawa, M., Morita, M., Einaga, K., Tanaka, K., Tanabe, T., Yamanouchi, K., Hirama, M., Tait, J.F. and Fujikawa, K. (1996) Secretory production of recombinant urokinase-type plasminogen activator-annexin V chimeras in Pichia pastoris. Gene 177, 69-76.
290. Mohanraj, D., Olson, T. and Ramakrishnan, S. (1995) Expression of biologically active human vascular endothelial growth factor in yeast. Growth Factors 12, 17ö27.
291. Saelens, X., Vanlandschoot, P., Martinet, W., Maras, M., Neirynck, S., Contreras, R., Fiers, W. and Jou, W.M. (1999) Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin. Eur. J. Biochem. 260, 166-175.
292. Martinet, W., Saelens, X., Deroo, T., Neirynck, S., Contreras, R., Min Jou, W. and Fiers, W. (1997) Protection of mice against a lethal influenza challenge by immunization with yeast-derived recombinant influenza neuraminidase. Eur. J. Biochem. 247, 332-338.
293. Zhu, X., Wu, S. and Letchworth, G.J., 3rd (1997) Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenecity. Vaccine 15, 679-688.
294. Zhu, X., Wu, S. and Letchworth, G.J. (1999) A chimeric protein comprised of bovine herpesvirus type 1 glycoprotein D and bovine interleukin-6 is secreted by yeast and possesses biological activities of both molecules. Vaccine 17, 269-282.
295. Sugrue, R.J., Fu, J., Howe, J. and Chan, Y.C. (1997) Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles. J. Gen. Virol. 78, 1861-1866.
296. Cregg, J.M., Tschopp, J.F., Stillman, C., Siegel, R., Akong, M., Craig, W.S., Buckholz, R.G., Madden, K.R., Kellaris, P.A., Davis, G.R., Smiley, B.L., Cruze, J., Torregrossa, R., Velicelebi, G. and Thill, G.P. (1987) High level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. Bio/Technology 5, 479-485.
297. Chiou, H.L., Lee, T.S., Kuo, J., Mau, Y.C. and Ho, M.S. (1997) Altered antigenicity of "a" determinant variants of hepatitis B virus. J. Gen. Virol. 78, 2639-2645.
298. Eckart, M.R. and Bussineau, C.M. (1996) Quality and authenticity of heterologous proteins synthesized in yeast. Curr. Opin. Biotechnol. 7, 525-530.
299. Lal, S.K., Tulasiram, P. and Jameel, S. (1997) Expression and characterization of the hepatitis E virus ORF3 protein in the methylotrophic yeast, Pichia pastoris. Gene 190, 63-67.
300. Scorer, C.A., Buckholz, R.G., Clare, J.J. and Romanos, M.A. (1993) The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136, 111-119.
301. Peng, Y.C. and Acheson, N.H. (1997) Production of active polyomavirus large T antigen in yeast Pichia pastoris. Virus Res. 49, 41-47.
302. Bisaillon, M., Bergeron, J. and Lemay, G. (1997) Characterization of the nucleoside triphosphate phosphohydrolase and helicase activities of the reovirus lambda1 protein. J. Biol. Chem. 272, 18298-18303.
303. Bisaillon, M., Senechal, S., Bernier, L. and Lemay, G. (1999) A glycosyl hydrolase activity of mammalian reovirus sigma1 protein can contribute to viral infection through a mucus layer. J. Mol. Biol. 286, 759-773.
304. Wiles, A.P., Shaw, G., Bright, J., Perczel, A., Campbell, I.D. and Barlow, P.N. (1997) NMR studies of a viral protein that mimics the regulators of complement activation. J. Mol. Biol. 272, 253-265.
305. Danty, E., Briand, L., Michard-Vanhee, C., Perez, V., Arnold, G., Gaudemer, O., Huet, D., Huet, J.C., Ouali, C., Masson, C., Pernollet, J.C. (1999) Cloning and expression of a queen pheromone-binding protein in the honeybee: an olfactory-specific, developmentally regulated protein. J. Neurosci. 19(17):7468-75.
306. Fedosov, S.N., Berglund, L., Nexo, E., Petersen, T.E. (1999) Sequence, S-S bridges, and spectra of bovine transcobalamin expressed in Pichia pastoris.    J. Biol. Chem. 274(37):26015-20.
307. Byrne, M.P., Titball, R.W., Holley, J., Smith, L.A.(2000) Fermentation, purification and efficacy of a recombinant vaccine candidate against botulinum neurotoxin type F from Pichia pastoris. Protein Expr. Purif.19:327-337.

308. Potter, K.J., Zhang, W., Smmith, L.A., Meagher, M.M. (2000) Production and purification of the heavy chain fragment C of botulinum neurotoxin, serotype A, expressed in the methylotropic yeast Pichia pastoris.,Protein Expr. Purif. 19:393-402.

309. Ayra-Pardo, C., Motejo-Sierra, I.L., Vasquez-Padron, R.I., Garcia-Martinez, C. (1999) β-D-glucuronidase gene from Escheria coli is a functional reporter in the methylotropic yeast Pichia pastoris,Lett. Appl. Microbiol.28: 278-283 .

310. Beldarrain, A., Acosta, N., Mmontesinos, R., Mata, M., Cremata, J.. (2000) Characterization of Mucor pusillus rennin expressed in Pichia pastoris: enzymic, spectroscopic and calorimetric studies.Biotechnol. Appl. Biochem. 31: 77-84.

311. Berrin, J., Williamson, G., Puigserver, A., Chaix, J-C., McLauchlan, W., Juge, N.(2000) High-level production of recombinant fungal endo-β-1,4-xylanase in the methylotropic yeast Pichia pastoris. Protein Expr. Purif. 19: 179-187.

312. Bieszke, J., Spudich, E., Scott, K., Borkovich, K., Spudich,J. (1999) A eukaryotic protein, NOP-1, binds retinal to from an archaeal rhodopsin-like photochemically reactive pigment. Biochemistry 38: 14138-14145.

313. Dan, S. Marton, I., Dekel, M., Bravdo, B-A., He, S., Withers, S., Soseyov, O. (2000) Cloning, expression, characterization and nucleophile identification of family 3, Aspergillus niger β-glucosidase. J. Biol. Chem. 275: 4973-4980.

314. Gelo-Pujic, M., Kim, H., Butlin, M., Palmore, G. (1999) Electrochemical studies of a truncated laccase produced in Pichia pastoris Appl. Environ. Microbiol. 65(12): 5515-5521
315. Godbole, S., Decker, S, Nieves, R., Adney, W., Vinzant., T., Baker, J., Thomas, S., Himmel, M. (1999) Cloning and expression of Trichoderma reesei cellobiohydrolase I in Pichia pastoris. Biotechnol. Prog. 15: 828-833.

316. Koller, A., Valesco, J., Subramani, S. (2000) The CUP1 promoter of Saccharomyces cerevisiae is inducible by copper in Pichia pastoris. Yeast 16: 651-656.

317. Maras, M., Callewaert, N., Piens, K., Claeyssens,M., Martinet, W., Dewaele, S., Contreras, H., Dewerte, I., Penttila, M., Contreras, R. (2000) Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-α-D-mannosidase. J. Biotechnol. 77: 255-263.

318. Otterbein, L., Recore, E., Longhi, S., Asther, M., Moukha, S. (2000) Molecular coloning of the cDNA encoding laccase from Pycnoporus cinnabarinus I-937 and expression in Pichia pastoris Eur.J. Biochem.267: 1619-1625.

319. Pratap, J., Rajamohan, G., Dikshit, K.L. (2000) Characteristics of glycosylated streptokinase secreted from Pichia pastoris:enhanced resistance of SK to proteolysis by glycosylation. Appl. Microbiol. Biotechnol. 53: 469-475.

320. Rodriguez, E., Mullaney, E., Lei, X. (2000) Expression of the Aspergillus fumigatus phytase gene in Pichia pastoris and characterization of the recombinant enzyme. Biochem. Biophys.Res. Commun 268(2): 324-331.

321. Rotticci-Mulder, J., Gustavsson, M., Holmquist, M., Hult, K., Martinelle, M. (2001) Expression in Pichia pastoris of Candida antarctica lipase B and lipase B fused to a cellulose-binding domain. Protein Expr. Purif. 21: 386-392.

322. Screen, S and St.Leger, R. (2000) Cloning, expression, and substrate specificity of a fungal chymotrypsin. J. Biol. Chem. 275: 6689-6694.

323. Tang, B., Bannerjee, B., Greenberger, P., Fink, J., Kelly, K., Kurup, V. (2000) Antibody binding of deletion mutants of Asp f 2, the major Aspergillus fumigatus allergen. Biochem. Biophys. Res. Commun. 270(3): 1128-1135.

324. Thomson, L., Bates, S., Yamazaki, S., Arisawa, M., Aoki, Y., Gow, N. (2000) Functional characterization of the Candida albicans MNT1 mannosyltransferase expressed heterologously in Pichia pastoris J. Biol. Chem. 275(25): 18933-18938.

325. Whittaker, M., Kersten, P., Cullte., Whittaker, J. (1999) Identification of catalytic residues in glyoxal oxidase by targeted mutagenesis. J. Biol. Chem. 274: 36226-36232.

326. Bush, R., Snachez, B, Geisler, G. (1999) Molecular cloning of a major Alernaria alternata allergen, rAlt a 2. J. Allergy Clin Immunol 104(3): 655-671.

327. Boer, H., Teeri, T., Kolvula, A. (2000) Characterization of Trichoderma reecei cellobiohydrolase Cel7A secreted from pichia pastoris using two different promoters. Biotechnol. Bioeng. 69(5): 486-94.

328. Andersen, M., Busk, P., Svendsen, I., Moller, B.(2000) CytochromesP-450 from Cassava(Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogeinc glucosides linamarin and lotaustralin. J. Biol. Chem. 275(3): 1966-1975.

329. Beekwilder, J., Schipper, B., Bakker,P., Bosch, G., Jongsma, M. (2000) Characterization of potato proteinase inhibitor II reactive site mutants. Eur. J. Biochem. 267:1975-1984.

330. Boraston, T., McLean, B., Guarna, M., Amandaron-Alkw, E., Kilburn, D. (2001) A family 2a carbohydrate-binding module as an affinity tag for proteins produced in Pichia pastoris. Protein Expr. Purif. 21: 417-423.

331. Caravalho, M., d’Arcy-Lamenta, A., Roy-Macauley, H., Gariel., El Maarouf, H., Pham-Thi, A., Zuily-Fodil, Y. (2001) Aspartic protease in leaves of common bean(Phaseolus vulgaris L.) and cowpea(Vigna unguiculata L. Walp): enzymatic activity, gene expression and relation to drought susceptibility. FEBS Letters 492: 242-246.

332. Delano, J., Dombrowski, J., Ryan, C. (1999) The expression of tomato prostemin in Escherichia coli: a structural challenge. Protein Expr. Purif. 17: 74-82.

333 Feng, L., Kirsch, J.(2000) L-Vinylglycine is an alternative substrate as well as a mechanism-based inhibitor of 1-aminocyclopropane-1-carboxylate synthase. Biochemistry 39(10): 2436-2444.

334. Gonzalez, E., Monsalve, R., Puente, X., Villaba, M., Rodriguez, R. Assignment of the disulfide bonds of Ole e 1, a major allergen of olive tree pollen involved in fertilization. J. Peptide Res. 55(1): 18-23.

335. Grobe, K., Becker, W-M, Schlaak, M., Petersen, A. (1999) Grass group I allergens(β expansins) are novel, papain-related proteinases. Eur.J. Biochem. 263(1): 33-40.

336. Kristensen, A., Brunstedt, J., Nielsen, J., Mikkelsen, J. Roepstorff, P., Nielsen, K.(1999) Processing, disulfide patern, and biological activity of a sugar beet defensin, AX2, expressed in Pichia pastoris. Protein Expr. Purif. 16(3): 377-387.

337. Koiwai, H., Akaba, S., Seo, M., Komano,T., Koshiba, T.(2000) functional expression of two Arabidopsis aldehyde oxidases in the yeast Pichia pastoris. J. Biochem.127(4):659-664.

338. Koyanagi, T., Matsumura, K., Kuroda, S., Tanizawa, K. (2000) Molecular cloning and heterologous expression of pea seedling copper amine oxidase. Biosci. Biotechnol. Biochem.64(4):717-722.

339. Kumagai, M., Donson, J., della-Cioppa,G., Grill, L. (2000) Rapid, high-level expression of glycoslyated rice α-amylase in transfected plants by an RNA viral vector. Gene 245(1): 169-74.

340. Maranville, E., Zhu.A. (2000) The carboxyl terminus of coffee bean α-galactosidase is critical for enzyme activity. Arch. Biochem.Biophys. 373(1): 75-85.

341. Maranville, E., Zhu, A.(2000) Assesssment of amino-acid substitutions at tryptophan 16 in α-galactosidase. Eur. J. Biochem. 267(5): 1495-1501.

342. Mertens, J., Shiraishi, N., Campbell, W. (2000) Recombinant espression of molybdenum reductase fragements of plant nitrate reductase at high levels in Pichia pastoris. Plant Physiol. 123k(2), 743-56.

343. Moreneo, J., David, N., Miernyk, J., Randall, D. (2000) Pisum sativum mitochondrial pyruvate dehydrogenase can be assembled as a functional α2β heterotetramer in the cytoplasm of Pichia pastoris. Protein Expr.Purif. 19(2): 276-83.

344. Morawski, B., Lin., Z., Cirino, P., Joo,H., Bandara, G., Arnold,F. (2000) Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Eng. 13(5): 377-84.

345. Muslin, E., Kanikula, A., Clark, S., Henson, H.(2000) Overexpression, purification and characterization of a barley α-glucosidase secreted by Pichia pastoris. Protein Expr. Purif. 18(1): 20-26.

346. Raemaekers, R, de Muro, L., Gatehouse, J., Fordham-Skelton, A.(1999) Functional phytohemagglutinin(PHA) and Galanthus nivalis agglutinin(GNA) expressed in Pichia pastoris. Eur. J. Biochem. 265(1): 394-403.

347. Ventrua, S., Villegas, V., Sterner, J., Larson,L., Vendrell, J., Hershberger, C., Aviles, F.(1999) Mapping the pro-region of carboxypeptidase B by protein engineering. J. Biol. Chem. 247(28):19925-33.

348. Volpicella, M., Schipper, A., Jongsma, M.. Spoto, N., Gallerani, R, Ceci, L. (2000) Characterization of recombinant mustard trypsin inhibitor 2 (MTI2) expressed in Pichia pastoris. FEBS Lett. 468(2-3): 137-141.

349. Rajamohan, F., Doumbia, S., Engstrom, C., Pendergras, S., Maher, D., Uckun, F. (2000) Expression of biologically active recombinant pokeweed antiviral protein in methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 18(2): 193-201.

350. Zhou, R. Kroczynska, B., Miernyk, J. (2000) Expression of the Arabidopsis thaliana AtJ2 cochaperone protein in Pichia pastoris. Protein Expr. Purif. 19(2): 253-258.

351. Boyle. S., Popp, M, Smith, W, Greenberg, R., James, M. (1998) Marine Environ. Res. 46(1-5): 25-28.

352. Caffrey, C., Mathieu, M, Gaffney, A, Salter, J, Sajid, M, Lucas, K., Franklin, Bogyo, M., McKerrow, J. (2000) Identifiaction of a cDNA encoding an active asparaginyl endopeptidase of Schistosoma mansoni and its expression in Pichia pastoris. FEBS Lett. 466: 244-248.

353. Garcia-Garcia, J., Montero, C., Redondo, M., Vargas, M., Canales, M., Boue, O., Rodriguez, M., Joglar, M., Machado, H., Gonzalez, I., Valdes, M., Mendez, L., Fuente, J.(2000) Control of ticks resistant to immunization with Bm86 in cttle vaccinated with the recombinant antigen Bm95 isolated from the cattle tick Boophilus microplus. Vaccine 18: 2275-2287.

354. Hussein, A., Smith, A., Chacon., Selkirk, M. (2000) Determinants of substrate specificity of a second non-neuronal secreted acetylcholinesterase from the parasitic nemaode Nippostrongylus brasiliensis. Eur. J. Biochem. 267: 2276-2282.

355. Jackson, V., Cameron, J., Zammit, V., Price, N. (1999) Sequencing and functional expression of the malonyl-CoA-sensitive carnitine palmitoyl transferase from Drosophila melanogaster. Biochem J. 341:483-489.

356. Keegan, K., Gerber, A., Brindel., Leemans., Gallo.,a., Keller, W., O’Connell, M. (2000) The properties of a tRNA-Specific adenosine deaminase from Drosophila melanogaster support an evolutionary link between pre-mRNA editing and tRNA modification. Mol. Cell Biol. 20(3): 825-833.

357. Koiwa, H., D’Urzo, M., Zhu-Salzman, K., Ibeas, J., Shade, R., Murdock., L., Bressan, R., Hasegawa, P.(2000) An in-gel assay of a recombinant western corn rootworm(Diabrotica virgifera virgifera) cysteine proteinase expressed in yeast. Anal. Biochem 282: 153-155.

358. Kristjansdottir, S., Gudmundsdottir, A. (2000) Propeptide dependent activation of the Antarctic krill euphauserase precursor produced in yeast. Eur. J. Biochem. 267:2632-2639.

359. Levandoski, M., Caffery, P., Rogowski, R., Lin, Y., Shi, Q-L., Hawrot, E. (2000) Recombinant expression of α-bungarotoxin in Pichia pastorisfacilitates identification of mutant toxins engineered to recognize neuronal nicotinic acetylcholine receptors. J. Neurochem. 74(3): 1279-1289.

360. Maeda, Y., Kuroki, R., Suzuki, H., Reilander, H. (2000) High-level secretion of biologically active recombinant human macrophage inflammatory protein-1α by the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 18(1), 56-63.

361. Monsalve, R., Lu, G., King, T. (1999) Expressions of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp(Polistes annularis), in bacteria or yeast. Protein Expr. Purif. 16(3): 410-416.

362. Munshi, C., Thiel,D., Mathews,I., Aarhus, R., Walseth, T., Lee, H. (1999) characterization of the active site of ADP-ribosyl cyclase. J. Biol.Chem. 274(43): 30770-30777.

363. Palladino, M., Keegan, L., O’Connell, M., Reenan,.(2000) dADAR, a Drosophila double-stranded RNA-specific adenosine deaminase is highly developmentally regulated and is itself a target for RNA editing. RNA 6(7): 1004-1018.

364. Briand, L., Nespoulous, C., Perez, V., Remy, J,., Huet, J., Pernollet. (2000) Ligand-binding properties and structural characterization of a novel rat odorant-binnding protein variant. Eur.J. biochem 267: 3079-3089.

365. Caldas, Coelho, V., Rigden, D., Nexchich, G., Moro, A., Brigido, M. (2000) Design and synthesis of germline-based hemi-humanized single-chain Fv against the CD18 surface antigen. Protein Eng. 13(5): 353-360.

366. Cupit, P., Whyte, J., Porter, A., Browne, M., Holmes, S., Harris, W., Cunningham, C. (1999). Lett. Appl. Microbiol. 29: 273-277.

367. Duggan, B., Dyson, H., Wright, P.(1999) Inherent flexibility in a potent inhibitor of blood coagulation, recombinant nematode anticoagulant protein c2. Eur. J. Biochem. 265: 539-548.

368. Evin, G., LeBrocque, D., Culvenor, J., Galatis, D., Weidemann, A., Beyreuther, K., Masters, C., Cappai, R. (2000) Presenilin I expression in yeast lowers secretion of the amyloid precursor protein. Mol. Neurosci. 11(27): 405-408.

369. Fischer, R., Dorssard, J., Emans. N., Commandeur, U., Hellwig, S. (1999) Towards molecular farming in the future: Pichia pastoris- based production of single-chain antibody fragments. Biotechnol. Appl. Biochem. 30:117-120.

370. Foss, D., Moody, M., Murphy, K., Pazmany, C., Zilliox, M., Murtaugh, M.(1999) In vitro and in vivo bioactivity of single-chain interleukin-12. Scand. J. Immunol. 50(6):596-604.

371.Freyre, F., Vazquez, J., Ayala, M., Canaan-Haden, L., Bell, H., Rodriguez, I., Gonzalez, A., Cintado, A., Gavilondo, J. (2000) Very high exprssion of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris. J. Biotechnol. 76(2-3): 157-163.

372. Goel, A., Beresford, G., Colcher, D., Pavlinkova, G., Booth, B., Baranowska-Kortylewicz, J., Batra, S. (2000) Divalent forms of CC49 single-chain antibody constructs in Pichia pastoris: Expression, purification and characterization. J. Biochem(Tokyo) 127(5): 829-836.

373. Jackson, V., Cameron, J., Fraser, F., Zammit, V., Price, N.(2000) Use of six chimeric proteins to investigate the role of intramolecular interactions in determining the kinetics of carnitine palmitoyltransferase I isoforms. J. biol. Chem. 275(26): 19560-19566.

374. Julien, M., Kajiji, S., Kaback, R., Gros, P. (2000) Simple purification of highly active biotinylated p-glycoprotein: enantiomer-specific modulation of drug-stimulated ATPas activity. Biochemistry 39(1): 75-85.

375. Kotti, T., Savolainen, K., Helander, H., Yagi, A., Novikov, D., Kalkkinen, N., Conzelmann, E., Hiltlunen, J., Schmitz, W. (2000) In mouse α-methylacyl-CoA racemase, the same gene product is simultaneously located in mitochondria and peroxisomes. J. Biol. Chem 275(27): 20887-20895.

376. Lerner-Marmarosh, N., Gimi, K., Urbatsch, I., Gros, P., Senior, A. 1999. Large-scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and charaterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins. J. Biol. Chem. 274(49): 34711-34718.

377. Li, Z., Xiong, F., Lin, Z., d’Anjou, M., Daugulis, A., Yang, D., Hew, C. (2001) Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris. Protein Expr. Purif. 21: 438-445.

378. Markaryan, A., Morozova, I., Lee, B., Kaplan, A. (1999) Atypical processing of amyloid precursor fusion protein by proteolytic activity in Pichia pastoris. Biochem. Biophys. Res. Comm. 262(1): 263-268.

379. Palczewska, M., Groves, P., Kuznicki, J. (1999) Use of Pichia pastoris for the exprssion, purification, and characterization of rat calretinin “EF-Hand” domains. Protein Expr. Purif. 17(3): 465-476.

380. Pru, J., Austin, K., Perrry, D., Nighswonger, A., Hansen, T. (2000) Production, purification and carboxy-tereminal sequencing of bioactive recombinant bovine interferon-stimulated gene product 17. Biol. Reprod. 63(2): 619-628.

381. Quigg, R., He, C., Hack, B., Alexander, J., Morgan, B.(2000) Production and functional analysis of rat CD59 and chimeric CD590-Crry as active soluble proteins in Pichia pastoris. Immunology 99(1): 46-53.

382. Rees, G., Gee, C., Ward, H., Ball, C., Tarrant, G., Poo, S., Bristow, A. (1999) Rat tumour necrosis factor-α: expression in recombinbant Pichia pastoris, purification, characterization and development of a novel ELISA. Eur. Cytokine Netw. 10(3): 383-92.

383. Urbatsch, I., Gimi, K., Wilke-Mounts, S., Senior, A. (2000) Conserved Walker A ser residues in the catalytic sites of p-glycoprotein are critical for catalysis and involved primarily at the transition state step. J. Biol. Chem. 275(32): 25031-25038.

384. Ventura, S., Villegas, V., Sterner, J., Larson, J., Vendrell, J., Hershberger, C., Aviles, F. (1999) Mapping the pro-region of carboxypeptidase B by protein engineering. J. Biol. Chem. 274(28): 19925-19933.

385. Werten, M., van den Bosch, T., Wind, R., MooiBroek, H., de Wolf, F. (1999) High yield secretion of recombinant gelatins by Pichia pastoris. Yeast 15(11): 1087-1096.

386. Woldegiorgis, G., Shi, J., Zhu, H., Arvidson, D. (2000) Functional characterization of mammalian mitochondrial carnitine palmitoyltransferases I and II expressed in the yeast Pichia pastoris. J. Nutr. 130(2S suppl): 310S-314S.

387. Kalidas, C., Joshi, L., Batt. (2001) Characterization of glycosylated variants of β-lactoglobin expressed in Pichia pastoris. Prot. Eng. 14(2): 201-207.

388. Stanssens, P., Bergum, P., Gansemans, Y., Jespers, L., Laroche, Y., Huang, S., Maki, S., Messens, J., Lauwereys, M., Cappello, M., Hotez, P., Lasters, I., Vlasuk, G. (1996). Anticoagulant repertoire of the hookworm Ancylostoma caninum. Proc. Natl. Acad. Sci., USA. 93:2149-2154.

389. Schmidt-Dannert, C. (1999) Recombinant microbial lipases for biotechnological applications. Bioorg. Med. Chem. 7: 2123-2130.

390. Nguye, T., Zasim, M., Morris, S., Lu, C., Hill, D., Laskowski, M., Sakanari, J. (1999) Expression and characterization of elastase inhibitors from the ascarid nematodes Anisakis simples and Ascaris suum. Mol. Biochem. Parisitol. 102: 79-89.

391. Quiralte, J., Gonzalez, E., Arias de Saavedra, J., Villalba, M., Florido, J., Saenz de San Pedro, B., Rodriguez, R. (2000) Immunological acrtivity of recombinant Ole e 1 in patients with Olea europaea pollinosis. Int Arch Allergy Immun 122: 101-107

392. Sakuma, Y., Kimura, M., Takabatake, T., Takeshima, K., Fujimura, H. Expression and secretion of a biologically active mouse hedgehog protein by the methylotropic yeast Pichia pastoris. (1999) Appl Microbiol Biotechnol 52: 410-414.

393. Ree, R., van Leeuwen, A., Bulder, I., Bonc, J., Aalberse, R. (1999) Purified natujral and recombinant Fel d 1 and cat albumin in in vitro diagnostics for cat allergy. J. Allergy. Clin. Imunol. 104(6): 1223-1229.

394. Ross, R., Foss, X., Ghadially, H., Lahr, T., Schwing, J., Knop, J., Reske-Kunz, A. (1999) Mouse langerhans cells differentially express an activated T cell-attracting CC chemokine. J Invest.Dermtaol. 113(6): 991-998.

395.Fisher, W., Greenwald, J., Park, M., Craig, A., Choe, S., Vale, W. (1999) The difulfide bond arrangement in the extracellular domain of the activin type II receptor. J. Protein Chem. 18(4): 437-446.

396. Blum, A., Martin, H., Maser, E. (2000) Human 11β-hydroxysteroid dehydrogenase 1/carbonyl reductase: recombinant expression in the yeast Pichia pastoris and Escherichia coli. Toxicology 144: 113-120.

397. Boehm, T., Pirie-Shepherd, S., Trinh, L., Shiloach, J., Folkman, J. (1999) Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin. Yeast 15: 563-572.

398. Brierly, R. Secretion of recombinant human insulin–like growth factor 1(IGF-1). In: Pichia Protocols(Meth. Mol. Biol. Vol. 103), D.R. Higgins and J. Cregg, eds. Humana, 1998.

399. Caputo, E., Carratore, V., Ciullo, M., Tiberio, C., Mani, J., Piatier-Tonneau, D., Guaridola, J. (1999) Biosynthesis and immunobiochemical characterization of gp17/GCDFP-15, a glycoprotein from seminal vesicles and from breast tumors, in HeLa cells and in Pichia pastoris yeast. Eur.J. Biochem. 265:664-670.

400. Chadd, H., Chamow, S. (2001) Therapeutic antibody expression technology. Curr. Opin. Biotechnol. 12(2)L 188-194.

401. Chotigeat, W., Chanunnukul, W., Phongdara, A. Expression of a mammalian α2,6-sialyltransferase gene in Pichia pastoris. J. Biotechnol. 81:55-61.

402. Dale, G., D’Arcy, B., Yuvaniyama, C., Wipf, B., Oefner, C., D”Arcy, A. (2000) Purification and crystallization of the extracellular domain of human neutral endopeptidase(neprilysin) expressed in Pichia pastoris. Acta Crystallographica D56: 894-897.

403. Dall’Acqua, W., Halin, C., Rodrigues, M., Carter, P. (1999) Elastase substrate specificity tailored through substrate-assisted catalysis and phage display. Protein Eng. 12(11):981-994.

404. Dockal, M., Carter, D., Rucher, F. (1999) The three recombinant domains of human serum albumin. J. Cell Biol. 274(41):29303-29310.

405. Goda, S., Takano, G., Yumagata, Y., Katakura, Y., Yutani, K. (2000) Effect of extra N-terminal residues on the stability and folding of human lysozyme expressed in Pichia pastoris. Protein Eng. 13(4): 299-307.

406. Hellwig, S., Robin, F., Drossard, J., Raven, N., Vaquero-Martin, C., Shively, J., Fisher, R. (1999) Production of carcinoembryonic antigen (CEA) N-A3 domain in Pichia pastoris by fermentation . Biotechnol. Appl. Biochem. 30: 267-275.

407. Kirkitadze, M., Dryden, D., Kelly, S., Price, N., Wang, X., Krych, M., Atkinson, J., Barlow, P.(1999) Co-operativity between modules within a C3b-binding site of complement receptor type 1. FEBS Lett. 459:133-138.

408. Kobayashi, K., Nakamura, N., Sumi, A., Ohmura, T., Yokoyama, K. (1998). The development of recombinant human serum albumin. Theraputic Apheresis 2(4): 257-262.

409. Johnson, T., Holaday, S., Sun, Y., Subramaniam, P., Johnson, H., Krishna, N.(1999) Expression, purification and characterization of interferon-τ produced in Pichia pastoris grown in a minimal medium. J. Interferon Cytokine Res. 19: 631-636.

410. Keizer-Gunnick, I., Vuorela, A., Myllyharju, J., Pihlajaniemi, T., Kivirikko, K., Veenhuis, M. (2000) Accumulation of properly folded type III procollagen molecules in specific intracellular membranous compartments in the yeast Pichia pastoris. Matrix Biology 19:29-36.

411. Kroll, K., Otte, S., Hirschfeld, G., barnikil-Watanabe, S., Gotz, H., Sternbach, H., Kratzin, H., Barnikil, H., Hilschmann, N. (1999) Heterologous overexpression of human NEFA and studies on the two EF-hand calcium-binding sites. Biochem. Biophys. Res. Comm. 260: 1-8.

412. Lee, K., Tae, W-C., Jackson, K., Kwon, S., McKee, P. (1999) Characterization of wild-type and mutant α₂ –antiplasmins: fibrionlysis enhancement by reactive site mutant. Blood 94(1): 164-171.

413. Leber, A., Hemmens, B., Klosh, B., Goessler, W. Raber, G., Mayer, B., Schmidt, K. (1999) Characterization of recombinant human endothelial nitric-oxide synthase purified from the yeast Pichia pastoris. J. Biol. Chem 274(52), 37658-37664.

414. Majerle, A., Kidric, J., Jerala, R. (1999) Expression and refolding of functional fragments of the human lipopolysaccharide receptor CD14 in Escherichia coli and Pichia pastoris. Protein Expr. Purif. 17:96-104.

415. Malissard, M., Zeng, S., Berger, E. (2000) Expression of functional suluble forms of human β-1,4-galactosyltransferase I, α-2,6 sialyltransferase and α-1,3-fucosyltransferase VI in the methylotrophic yeast Pichia pastoris. Biochem. Biophys. Res. Comm. 267:169-173.

416. Moritz, R., Ward, L., Tu, G., Fabri, L., Hong, J., Yasukawa, K., Simpson, R. (1999) The N-terminus of gp130 is critical for the formation of the high-affinity interleukin-6 receptor complex. Growth Factors 16:265-278.

417. Nagler, D., Zhang, R., Tam, W., Sulea, T., Purisima, E., Menard, R. (1999) Human cathepsin X: a cysteine protease with unique carboxypeptidase activity. Biochemistry 38: 12648-12654.

418. Nakakubo, H., Fukayama, H., Nakajima, M., Imada, T., Uno,S., Shiota, N., Takai, S., Miyazaki, M, Nakamura, N. (2000) Secretory production of recombinant human chymase as an active form in Pichia pastoris. Yeast 16: 315-323.

419. Nilsen, S., Prorok, M., Castellino, F. (1999) Enhancement through mutagenesis of the binding of the isolated kringle 2 domain of human plasminogen to ω-amino acid ligands and to an internal sequence of a Streptoccal surface protein. J. Biol. Chem. 274(32): 22380-22386.

420. Oka, C., Tanaka, M., Muraki, M., Harata, K., Suzuki, K., Jigami, Y. (1999) Human lysozyme secretion increased by alpha-factor pro-sequence in Pichia pastoris. Biosci. Biotechnol. Biochem. 63(11): 1977-1983.

421. Ouzzine, M., Gulberti, S., Netter, P., Magdalou, J., Fournel-Gigleux, S. (2000) Structure/function of the human galβ1,3-glucornosyltransferase. J. Biol. Chem 275(36): 28254-28260.

422. Ouzzine, M., magdalou, J., Burchell, B., Fournel-Gigleux, S. (1999) An internal signal sequence mediates the targeting and retention of the human UDP-glucoronosyltransferase 1A6 to the endoplasmic reticulum. J. Biol. Chem. 274(44): 31401-31409.

423. Roderfeld, M., Buttner, F., Bartnik, E., Tschesche, H. (2000) Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris. Protein Expr. Purif. 19: 369-374.

424. Rogelj, B., Strukelj, B., Bosch, D., Jongsma, M. (2000) Expression , purification and characterization of equistatin in Pichia pastoris. Protein Expr. Purif. 19:329-334.

425. Roy, N., Padmanabhan, S., Smith, M., Shi, L., Navre, M., Das, G. (1999)Expression of human gelatinase B in Pichia pastoris. Protein Expr. Purif. 16: 324-330.

426. Petersen, C., Ha, C., Harohalli, K., Park, D., Feix, J., Isozaki, O., Bhagavan, N. (1999) Structural investigations of a new familial dysalbuminemic hyperthyroxinemia genotype. Clin. Chem.45(8): 1248-1254.

427. Sen Gupta, C., Dighe, R. (2000) Biological activity of single chain chorionic gonadotropin, hCGαβ, is decreased upon deletion of five carboxyl terminal amino acids of the α subunit without affecting its receptor binding. J. Mol. Endocrinol. 24:57-164.

429. Shang, J., Qiu, R., Wang, J., Liu, J., Zhou, R., Ding, H., Yang, S., Zhang, S., Jin, C. (1999) Molecular cloning and expression of galβ1,3 galNAc α2,3-sialyltransferase from human fetal liver. Eur. J. Biochem. 265: 580-588.

430. Shi, J., Zhu, H., Arvidson, D., Woldegiorgis, G.(2000) the first 28 N-terminal amino acid residues of human heart muscle carnitine palmitoyltransferase I are essential for malonyl CoA sensitivity and high-affinity binding. Biochemistry 39: 712-717.

431. Sieg, S., Smith, D., Kaplan, D. Differential activity of soluble versus cellular fas ligand: regulation by an accessory molecule. Cell. Immunol. 195:89-95.

432. Sun, J., Bird, C., Buzza, M., McKee, K., Whisstock, J., Bird, P. (1999) Expression and purification of recombinant human granzyme B from Pichia pastoris. Biochem. Biophys. Res. Comm. 261: 251-255.

433. Swiatek, W., Sugajska, E., Lankiewicz, L., Hammings, B., Zolnierowicz, S. (2000) biochemical characterization of recombinant subunits of type 2A protein phosphatase overexpressed in Pichia pastoris. Eur. J., Biochem. 267: 5209-5216.

434. Thielens, N., Enrie, K., Lacroix, M., Jaquinod, M., Hernandez, J., Esser, A., Arlaud, G. (1999) The N-terminal CUB-epidermal growth factor module pair of human complement protease C1r binds Ca²⁺ with high affinity and mediates Ca²⁺-dependent interaction with C1s. J. Biol. Chem. 274(14): 9149-9159.

435. Thoden, J., Wohlers, T., Fridovich-Keil, J., Holden, H. (2000) Crystallographic evidence for tyr 157 functioning as the active site base in human UDP-galactose 4-epimerase. Biochemistry 39: 5691-5701.

436. Truneh, A., Sharma, S., Silverman, C., Khandekar, S., Reddy, M., Deen, K., Mclaughlin, M., Srinivasula, S., Livi, G., Marsahhl, L., Alnemri, E., Williams, W., Doyle, M. (2000) Temperature-sensitive differential affinity of TRAIL for its receptors. J. Biol. Chem. 275(30): 23319-23325.

437. Vuorela, A., Mylllyharju, J., Pihlajaniemi, T., Kivirikko, K. (1999) Co expression with collagen markedly increases the half-life of the recombinant human prolyl 4-hydroxylase tetramer in the yeast Pichia pastoris. Matrix Biology 18: 519-522.

438. Williams, K., Rayhorn, P., Chi-Rosso, G., Garber, E., Strauch, K., Horan, G., O.Reilly, J., Baker, D., Taylor, F., Kotieliansky, V., Pepinsky, R.(1999). Functional antogonists of sonic hedgehog reveal the importance of the N terminus for activity. J. Cell Sci. 112: 4405-4414.

439. Zhan, H., Liu, B., Reid, S., Aoki, K., Li, C., Syed, R, Karkaria, C., Koe, G., Sitney, K., Hayenga, K., Mistry, F., Savel, L., Dreyer, M., Katz, B., Schreurs, J., Matthews, D., Cheetham, C., Egrie, J., Giebel, L., Stroud, R., Engineering a soluble extracellular erythropopietin receptor(EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythrypoietin. Protein Eng. 12(6): 505-513.

440. Falcon, V., Garcia, C., de la Rosa, M., Menendez, I., Seoane, J., Grillo, J. (1999) Ultrastructural and immunocytochemical evidences of core-particle formation in the methylotrophic Pichiia pastoris yeast when expression HCV structural proteins(core-E1). Tissue & Cell 31(2): 117-125.

441. Hardy, E., Martinez, E., Diago, D., Diaz, R., Gonzalez, D., Herrera, L. (2000) Large-scale production of recombinant hepatitis B surface antigen from Pichia pastoris. J. Biotechnol. 77: 157-167.

442. Hwang, J., Yamada, K., Honda, A., Nakade, K., Ishihama, A. Expression of functional influenza virus RNA polymerase in the methylotrophic yeast Pichia pastoris. J. Virol. 74(9): 4074-4084.

443. Kirkitadze, M., Henderson, C., Price, N., Kelly, S., Mullin, N., Parkinson, J., Dryden, D., Barlow, P. Central modules of the vaccinia virus complement control protein are not in extensive contact. Biochem. J., 344: 167-175.

444. Lal, S., Kumar, V. (1999) The X gene of hepatitis B virus shows a high level stimulation of the Rous sarcoma virus long terminal repeat in the methylotropic yeast, Pichia pastoris. FEBS Letters 456: 108-112.

445. Peeters, B., LeeuW, O., Verstegen, I., Koch, G., Gielkens, A.(2001) Generation of a recombinant chemeric Newcastle disease virus vaccine that allows serological differentiation between vaccinated and infected animals. Vaccine 19: 1616-1627.

446. Quinkal, I., Hernandez, J., Chevallier, S., Arlaud, G., Vernet, T.(1999) Mapping of the interaction between the immunodominant loop of the ectodomain of HIV-1gp41 and human complement protein C1q. Eur. J. Biochem. 265: 656-663.

447. Wen, J., Kinnear,M., Richardson, M., Willetts, N., Russell-Jones, G., Gordon, M., Alpers, D.(2000) Functional expression in Pichia pastoris of human and rat intrinsic factor.Biochem. Biophys. Acta 1490(1-2): 43-53.

448. Milek, R., Stunnenberg, S., Konings, R. (2000) Assembly and expression of a synthetic gene encoding the antigen Pfs48/45 of the human malaria parasite Plasmodium falciparum in yeast. Vaccine 18: 1402-1411.

449. Withers-Martinez, C., Carpenter, E., Hackett, F., Ely, B., Sajid, M., Grainger, M., Blackman, M. (1999) PCR-based gene synthesis as an efficient approach for expression of the A+T-rich malaria genome. Protein Engineering 12(12): 1113-1120.

450. Lefkowitz, L.J., Shinohara, H., Dennis, E.A. (2000) Human Group-V phospholipase-A₂ expression in Pichia pastoris and its role in eicosanoid generation. Ernst Schering Res. Found. Workshop (31) 25-52.